{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:06:43Z","timestamp":1791288403659,"version":"4.3.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T00:00:00Z","timestamp":1149638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,6,15]]},"abstract":"<jats:p>Earlier studies indicate a crucial role for the interconnected network of intrinsically bursting CA3 pyramidal neurons in the generation of <jats:italic>in vivo<\/jats:italic> hippocampal sharp waves (SPWs) and their proposed neonatal <jats:italic>in vitro<\/jats:italic> counterparts, the giant depolarizing potentials (GDPs). While mechanisms involving ligand\u2010 and voltage\u2010gated channels have received lots of attention in the generation of CA3 network events in the immature hippocampus, the contribution of ion\u2010transport mechanisms has not been extensively studied. Here, we show that bumetanide, a selective inhibitor of neuronal Cl<jats:sup>\u2212<\/jats:sup> uptake mediated by the Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup>\u20132Cl<jats:sup>\u2212<\/jats:sup> cotransporter isoform 1 (NKCC1), completely and reversibly blocks SPWs in the neonate (postnatal days 7\u20139) rat hippocampus <jats:italic>in vivo<\/jats:italic>, an action also seen on GDPs in slices (postnatal days 1\u20138). These findings strengthen the view that GDPs and early SPWs are homologous events. Gramicidin\u2010perforated patch recordings indicated that NKCC1 accounts for a large (\u223c10 mV) depolarizing driving force for the GABA<jats:sub>A<\/jats:sub> current in the immature CA3 pyramids. Consistent with a reduction in the depolarization mediated by endogenous GABA<jats:sub>A<\/jats:sub>\u2010receptor activation, bumetanide inhibited the spontaneous bursts of individual neonatal CA3 pyramids, but it slightly increased the interneuronal activity as seen in the frequency of spontaneous GABAergic currents. An inhibitory effect of bumetanide was seen on the <jats:italic>in vitro<\/jats:italic> population events in the absence of synaptic GABA<jats:sub>A<\/jats:sub> receptor\u2010mediated transmission, provided that a tonic GABA<jats:sub>A<\/jats:sub> receptor\u2010mediated current was present. Our work indicates that NKCC1 expressed in CA3 pyramidal neurons promotes network activity in the developing hippocampus.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.107086","type":"journal-article","created":{"date-parts":[[2006,4,28]],"date-time":"2006-04-28T00:30:10Z","timestamp":1146184210000},"page":"765-773","source":"Crossref","is-referenced-by-count":124,"title":["The cation\u2010chloride cotransporter NKCC1 promotes sharp waves in the neonatal rat hippocampus"],"prefix":"10.1113","volume":"573","author":[{"given":"Sampsa 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