{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T03:25:21Z","timestamp":1778556321662,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T00:00:00Z","timestamp":1152144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,7,15]]},"abstract":"<jats:p>Gonadotropin\u2010releasing hormone (GnRH) neurones play an essential role in the hypothalamo\u2010pituitary\u2010gonadal axis. As for other neurones, the discharge pattern of action potentials is important for GnRH neurones to properly function. In the case of a luteinizing hormone (LH) surge, for example, GnRH neurones are likely to continuously fire for more than an hour. For this type of firing, GnRH neurones must have a certain intrinsic property. To address this issue, we investigated the voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> currents and Ca<jats:sup>2+<\/jats:sup>\u2010activated voltage\u2010independent K<jats:sup>+<\/jats:sup> currents underlying afterhyperpolarization, because they affect cell excitability. Dispersed GnRH neurones from adult GnRH\u2010EGFP (enhanced green fluorescent protein) transgenic rats were cultured overnight and then used for an electrophysiological experiment involving the perforated patch\u2010clamp configuration. The GnRH neurones showed five subtypes of voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> currents, i.e. the T\u2010, L\u2010, N\u2010, P\/Q\u2010 and R\u2010types. The GnRH neurones also showed a slow afterhyperpolarization current (<jats:italic>I<\/jats:italic><jats:sub>sAHP<\/jats:sub>), but not a medium one. It is reported that the SK channel blocker apamin (10 p<jats:sc>m<\/jats:sc>\u2013100 n<jats:sc>m<\/jats:sc>) blocks a medium afterhyperpolarization current but not an <jats:italic>I<\/jats:italic><jats:sub>sAHP<\/jats:sub>. In contrast to previous reports, the <jats:italic>I<\/jats:italic><jats:sub>sAHP<\/jats:sub> observed in rat GnRH neurones was potently blocked by apamin. In addition, the GnRH neurones expressed transcripts for SK1\u20133 channels. The results indicate that rat GnRH neurones express all five subtypes of voltage\u2010gated Ca<jats:sup>2+<\/jats:sup> channels and exhibit an apamin\u2010sensitive <jats:italic>I<\/jats:italic><jats:sub>sAHP<\/jats:sub>, which may allow continuous firing in response to a relatively strong depolarizing input.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.110155","type":"journal-article","created":{"date-parts":[[2006,4,21]],"date-time":"2006-04-21T00:36:39Z","timestamp":1145579799000},"page":"431-442","source":"Crossref","is-referenced-by-count":44,"title":["The SK channel blocker apamin inhibits slow afterhyperpolarization currents in rat gonadotropin\u2010releasing hormone 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