{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:04:47Z","timestamp":1790809487319,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T00:00:00Z","timestamp":1158883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p>Intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) is a key factor controlling secretion from various cell types. We investigated how different patterns of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> signals evoke salt secretion <jats:italic>via<\/jats:italic> ion transport mechanisms and mucin secretion <jats:italic>via<\/jats:italic> exocytosis in dog pancreatic duct epithelial cells (PDEC). Activation of epithelial P2Y<jats:sub>2<\/jats:sub> receptors by UTP generated two patterns of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> change: 2\u201310 \u03bc<jats:sc>m<\/jats:sc> UTP induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations, whereas 100 \u03bc<jats:sc>m<\/jats:sc> UTP induced a sustained [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase, both in the micromolar range. As monitored by carbon\u2010fibre amperometry, the sustained [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase stimulated a larger increase in exocytosis than [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations, despite their similar amplitude. In contrast, patch\u2010clamp recordings revealed that [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations synchronously activated a K<jats:sup>+<\/jats:sup> current as efficiently as the sustained [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increase. This K<jats:sup>+<\/jats:sup> current was mediated by intermediate\u2010conductance Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> channels (32 pS at \u2212100 mV) which were sensitive to charybdotoxin and resistant to TEA. Activation of these Ca<jats:sup>2+<\/jats:sup>\u2010dependent K<jats:sup>+<\/jats:sup> channels hyperpolarized the plasma membrane from a resting potential of \u221240 mV to \u221290 mV, as monitored in perforated whole\u2010cell configuration, in turn enhancing Na<jats:sup>+<\/jats:sup>\u2010independent, Cl<jats:sup>\u2212<\/jats:sup>\u2010dependent and DIDS\u2010sensitive HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> secretion, as monitored through changes in intracellular pH. PDEC therefore encode concentrations of purinergic agonists as different patterns of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> changes, which differentially stimulate K<jats:sup>+<\/jats:sup> channels, the Cl<jats:sup>\u2212<\/jats:sup>\u2013HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchanger, and exocytosis. Thus, in addition to amplitude, the temporal pattern of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increases is an important mechanism for transducing extracellular stimuli into different physiological effects.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.114876","type":"journal-article","created":{"date-parts":[[2006,7,21]],"date-time":"2006-07-21T00:24:43Z","timestamp":1153441483000},"page":"163-178","source":"Crossref","is-referenced-by-count":27,"title":["Pattern of Ca<sup>2+<\/sup> increase determines the type of secretory mechanism activated in dog pancreatic duct epithelial cells"],"prefix":"10.1113","volume":"576","author":[{"given":"Seung\u2010Ryoung","family":"Jung","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungjin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertil","family":"Hille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toan D.","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duk\u2010Su","family":"Koh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,9,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(96)00013-2"},{"key":"e_1_2_5_3_1","first-page":"1473","volume-title":"Physiology of the Gastrointestinal Tract","author":"Argent BE","year":"1994"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019915"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1155"},{"key":"e_1_2_5_6_1","first-page":"G827","article-title":"Profound increase in viscosity and aggregation of pig gastric mucin at low pH","volume":"261","author":"Bhaskar KR","year":"1991","journal-title":"Am J Physiol"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1988.255.6.C844"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.25.4.4413"},{"key":"e_1_2_5_9_1","first-page":"L775","article-title":"Modulation of Cl\u2212 secretion by benzimidazolones. 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