{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T11:48:07Z","timestamp":1788608887598,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,10,4]],"date-time":"2006-10-04T00:00:00Z","timestamp":1159920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,10,15]]},"abstract":"<jats:p>Despite the importance of short\u2010chain fatty acids (SCFA) in maintaining the ruminant physiology, the mechanism of SCFA absorption is still not fully studied. The goal of this study was to elucidate the possible involvement of monocarboxylate transporter 1 (MCT1) in the mechanism of SCFA transport in the caprine rumen, and to delineate the precise cellular localization and the level of MCT1 protein along the entire caprine gastrointestinal tract. RT\u2010PCR revealed the presence of mRNA encoding for MCT1 in all regions of the caprine gastrointestinal tract. Quantitative Western blot analysis showed that the level of MCT1 protein was in the order of rumen \u2265 reticulum &gt; omasum &gt; caecum &gt; proximal colon &gt; distal colon &gt; abomasum &gt; small intestine. Immunohistochemistry and immunofluorescence confocal analyses revealed widespread immunoreactive positivities for MCT1 in the caprine stomach and large intestine. Amongst the stratified squamous epithelial cells of the forestomach, MCT1 was predominantly expressed on the cell boundaries of the stratum basale and stratum spinosum. Double\u2010immunofluorescence confocal laser\u2010scanning microscopy confirmed the co\u2010localization of MCT1 with its ancillary protein, CD147 in the caprine gastrointestinal tract. <jats:italic>In vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> functional studies, under the influence of the MCT1 inhibitors, <jats:italic>p<\/jats:italic>\u2010chloromercuribenzoate (pCMB) and <jats:italic>p<\/jats:italic>\u2010chloromercuribenzoic acid (pCMBA), demonstrated significant inhibitory effect on acetate and propionate transport in the rumen. This study provides evidence, for the first time in ruminants, that MCT1 has a direct role in the transepithelial transport and efflux of the SCFA across the stratum spinosum and stratum basale of the forestomach toward the blood side.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.115931","type":"journal-article","created":{"date-parts":[[2006,8,11]],"date-time":"2006-08-11T00:14:11Z","timestamp":1155255251000},"page":"635-647","source":"Crossref","is-referenced-by-count":94,"title":["Monocarboxylate transporter 1 (MCT1) plays a direct role in short\u2010chain fatty acids absorption in caprine 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