{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:59:47Z","timestamp":1791118787409,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T00:00:00Z","timestamp":1158883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p>5\u2010Hydroxytryptamine (5\u2010HT) evokes long\u2010term activation of neuronal activity in the nervous system. Carotid bodies, the sensory organs for detecting arterial oxygen, express 5\u2010HT. In the present study we examined whether 5\u2010HT evokes sensory long\u2010term facilitation (LTF) of the carotid body, and if so by what mechanism(s). Experiments were performed on anaesthetized adult rats and mice. Sensory activity was recorded from carotid bodies <jats:italic>ex vivo<\/jats:italic>. Spaced (3 \u00d7 15 s of 100 n<jats:sc>m<\/jats:sc> at 5 min intervals) but not mass (300 n<jats:sc>m<\/jats:sc>, 45 s) application of 5\u2010HT elicited LTF, whereas both modes of 5\u2010HT application evoked initial sensory excitation of the carotid bodies in rats. Ketanserin, a 5\u2010HT<jats:sub>2<\/jats:sub> receptor antagonist prevented sensory LTF but not the initial sensory excitation. Spaced application of 5\u2010HT activated protein kinase C (PKC) as evidenced by increased phosphorylations of PKC at Thr<jats:sup>514<\/jats:sup> and myristoylated alanine\u2010rich C kinase substrate (MARCKS) and these effects were abolished by ketanserin as well as bisindolylmaleimide (Bis\u20101), an inhibitor of PKC. Bis\u20101 prevented 5\u2010HT\u2010evoked sensory LTF. 5\u2010HT increased NADPH oxidase activity and PKC\u2010dependent phosphorylation of p47<jats:sup>phox<\/jats:sup> subunit of the oxidase complex. NADPH oxidase inhibitors (apocynin and diphenyl iodinium), as well as an anti\u2010oxidant (<jats:italic>N<\/jats:italic>\u2010acetyl cysteine), prevented 5\u2010HT\u2010evoked sensory LTF. Mice deficient in gp91<jats:sup>phox<\/jats:sup>, the membrane subunit of the NADPH oxidase complex, showed no sensory LTF, although responding to 5\u2010HT with initial afferent nerve activation, whereas both LTF and initial excitation by 5\u2010HT were seen in wild\u2010type mice. These results demonstrate that spaced but not mass application of 5\u2010HT elicits sensory LTF of the carotid body via activation of 5\u2010HT<jats:sub>2<\/jats:sub> receptors, which involves a novel signalling mechanism coupled to PKC\u2010dependent activation of NADPH oxidase.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.116020","type":"journal-article","created":{"date-parts":[[2006,8,4]],"date-time":"2006-08-04T00:26:00Z","timestamp":1154651160000},"page":"289-295","source":"Crossref","is-referenced-by-count":79,"title":["5\u2010HT evokes sensory long\u2010term facilitation of rodent carotid body via activation of NADPH oxidase"],"prefix":"10.1113","volume":"576","author":[{"given":"Ying\u2010Jie","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxiang","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank 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