{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:12:08Z","timestamp":1791162728705,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,1,26]],"date-time":"2007-01-26T00:00:00Z","timestamp":1169769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:p>The mammalian carotid body (CB) is a polymodal chemosensor which can detect low blood glucose (hypoglycaemia), leading to increased afferent discharge and activation of counter\u2010regulatory autonomic pathways. The underlying neurotransmitter mechanisms are unknown and controversy surrounds whether the action of low glucose is direct or indirect. To address this, we used a coculture model containing functional chemosensory units of rat CB receptor (type I) cell clusters and afferent petrosal neurones (PN). During perforated\u2010patch, whole\u2010cell recordings, low glucose (0\u20132 m<jats:sc>m<\/jats:sc>) stimulated sensory discharge in cocultured PN. When the background <jats:styled-content><jats:italic>P<\/jats:italic><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/tex2gif-sub-1.gif\" xlink:title=\"urn:x-wiley:00223751:media:TJP1962:tex2gif-sub-1\"\/><\/jats:styled-content> was lowered to levels typical of arterial blood (\u223c90 mmHg), robust PN chemoexcitation could be induced by physiological hypoglycaemia (3.3\u20134 m<jats:sc>m<\/jats:sc> glucose). These sensory responses were reversibly inhibited by a combination of purinergic (suramin, 50 \u03bc<jats:sc>m<\/jats:sc>) and nicotinic (mecamylamine, 1 \u03bc<jats:sc>m<\/jats:sc>) receptor blockers, suggesting that transmission depended on corelease of ATP and ACh. Hypoglycaemic responses were additive with those evoked by hypoxia or hypercapnia; further, they could be potentiated by the GABA<jats:sub>B<\/jats:sub> receptor blocker (CGP 55845) and inhibited by 5\u2010HT<jats:sub>2A<\/jats:sub> receptor blockers (ketanserin or ritanserin). During paired simultaneous recordings from a PN and a type I cell in an adjacent cluster, the afferent PN response coincided with type I cell depolarization, which was associated with a <jats:italic>decrease<\/jats:italic> in input resistance. In fresh tissue slices of rat CB, low glucose stimulated ATP secretion as determined by the luciferin\u2013luciferase assay; this secretion was cadmium sensitive, potentiated by CGP 55845, and inhibited by ketanserin. Taken together these data indicate that CB receptors act as direct glucosensors, and that processing of hypoglycaemia utilizes similar neurotransmitter and neuromodulatory mechanisms as hypoxia.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.121871","type":"journal-article","created":{"date-parts":[[2006,11,24]],"date-time":"2006-11-24T01:34:39Z","timestamp":1164332079000},"page":"735-750","source":"Crossref","is-referenced-by-count":83,"title":["Neurotransmitter mechanisms mediating low\u2010glucose signalling in cocultures and fresh tissue slices of rat carotid body"],"prefix":"10.1113","volume":"578","author":[{"given":"Min","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Buttigieg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin 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