{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:42:10Z","timestamp":1791164530214,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p>This study examines the molecular basis for the T\u2010type and L\u2010type Ca<jats:sup>2+<\/jats:sup> currents in canine Purkinje cells. The <jats:italic>I<\/jats:italic><jats:sub>CaT<\/jats:sub> in Purkinje cells was completely suppressed by 200 n<jats:sc>m<\/jats:sc> kurtoxin, a specific blocker of both Ca<jats:sub>v<\/jats:sub>3.1 and Ca<jats:sub>v<\/jats:sub>3.2 channels. Since only Ca<jats:sub>v<\/jats:sub>3.2 mRNA is expressed at high levels in Purkinje fibres, being approximately 100\u2010fold more abundant than either Ca<jats:sub>v<\/jats:sub>3.1 or Ca<jats:sub>v<\/jats:sub>3.3 mRNAs, it is concluded that the Ca<jats:sub>v<\/jats:sub>3.2 gene encodes the bulk of the T\u2010type Ca<jats:sup>2+<\/jats:sup> channels in canine Purkinje cells. This conclusion is consistent with the sensitivity of the current to blockade by Ni<jats:sup>2+<\/jats:sup> ions (<jats:italic>K<\/jats:italic><jats:sub>D<\/jats:sub>= 32 \u03bc<jats:sc>m<\/jats:sc>). For L\u2010type channels, Ca<jats:sub>v<\/jats:sub>1.2 mRNA was most abundant in Purkinje fibres but a significant level of Ca<jats:sub>v<\/jats:sub>1.3 mRNA expression was also found. A comparison of the sensitivity to blockade by isradipine of the L\u2010type currents in Purkinje cells and ventricular epicardial myocytes, which only express Ca<jats:sub>v<\/jats:sub>1.2, suggests that the Ca<jats:sub>v<\/jats:sub>1.3 channels make, at most, a minor contribution to the L\u2010type current in canine Purkinje cells.<\/jats:p>","DOI":"10.1113\/jphysiol.2006.127480","type":"journal-article","created":{"date-parts":[[2007,1,12]],"date-time":"2007-01-12T01:25:13Z","timestamp":1168565113000},"page":"465-471","source":"Crossref","is-referenced-by-count":28,"title":["Molecular basis of the T\u2010 and L\u2010type Ca<sup>2+<\/sup> currents in canine Purkinje 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