{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:36:48Z","timestamp":1791106608489,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,6,6]],"date-time":"2007-06-06T00:00:00Z","timestamp":1181088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,6,15]]},"abstract":"<jats:p>We determined the expression of Kv2 channel subunits in rat somatosensory and motor cortex and tested for the contributions of Kv2 subunits to slowly inactivating K<jats:sup>+<\/jats:sup> currents in supragranular pyramidal neurons. Single cell RT\u2010PCR showed that virtually all pyramidal cells expressed Kv2.1 mRNA and \u223c80% expressed Kv2.2 mRNA. Immunocytochemistry revealed striking differences in the distribution of Kv2.1 and Kv2.2 subunits. Kv2.1 subunits were clustered and located on somata and proximal dendrites of all pyramidal cells. Kv2.2 subunits were primarily distributed on large apical dendrites of a subset of pyramidal cells from deep layers. We used two methods for isolating currents through Kv2 channels after excluding contributions from Kv1 subunits: intracellular diffusion of Kv2.1 antibodies through the recording pipette and extracellular application of rStromatoxin\u20101 (ScTx). The Kv2.1 antibody specifically blocked the slowly inactivating K<jats:sup>+<\/jats:sup> current by 25\u201350% (at 8 min), demonstrating that Kv2.1 subunits underlie much of this current in neocortical pyramidal neurons. ScTx (300 n<jats:sc>m<\/jats:sc>) also inhibited \u223c40% of the slowly inactivating K<jats:sup>+<\/jats:sup> current. We observed occlusion between the actions of Kv2.1 antibody and ScTx. In addition, Kv2.1 antibody\u2010 and ScTx\u2010sensitive currents demonstrated similar recovery from inactivation and voltage dependence and kinetics of activation and inactivation. These data indicate that both agents targeted the same channels. Considering the localization of Kv2.1 and 2.2 subunits, currents from truncated dissociated cells are probably dominated by Kv2.1 subunits. Compared with Kv2.1 currents in expression systems, the Kv2.1 current in neocortical pyramidal cells activated and inactivated at relatively negative potentials and was very sensitive to holding potential.<\/jats:p>","DOI":"10.1113\/jphysiol.2007.128454","type":"journal-article","created":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T00:08:22Z","timestamp":1174608502000},"page":"941-960","source":"Crossref","is-referenced-by-count":103,"title":["Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neurons"],"prefix":"10.1113","volume":"581","author":[{"given":"D.","family":"Guan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Tkatch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. J.","family":"Surmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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