{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:57:01Z","timestamp":1790981821832,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,9,19]],"date-time":"2007-09-19T00:00:00Z","timestamp":1190160000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p>When olfactory receptor neurons respond to odours, a depolarizing Cl<jats:sup>\u2212<\/jats:sup> efflux is a substantial part of the response. This requires that the resting neuron accumulate Cl<jats:sup>\u2212<\/jats:sup> against an electrochemical gradient. In isolated olfactory receptor neurons, the Na<jats:sup>+<\/jats:sup>\u2013K<jats:sup>+<\/jats:sup>\u20132Cl<jats:sup>\u2212<\/jats:sup> cotransporter NKCC1 is essential for Cl<jats:sup>\u2212<\/jats:sup> accumulation. However, in intact epithelium, a robust electrical olfactory response persists in mice lacking NKCC1. This response is largely due to a neuronal Cl<jats:sup>\u2212<\/jats:sup> efflux. It thus appears that NKCC1 is an important part of a more complex system of Cl<jats:sup>\u2212<\/jats:sup> accumulation. To identify the remaining transport proteins, we first screened by RT\u2010PCR for 21 Cl<jats:sup>\u2212<\/jats:sup> transporters in mouse nasal tissue containing olfactory mucosa. For most of the Cl<jats:sup>\u2212<\/jats:sup> transporters, the presence of mRNA was demonstrated. We also investigated the effects of pharmacological block or genetic ablation of Cl<jats:sup>\u2212<\/jats:sup> transporters on the olfactory field potential, the electroolfactogram (EOG). Mice lacking the common Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchanger AE2 had normal EOGs. Block of NKCC cotransport with bumetanide reduced the EOG in epithelia from wild\u2010type mice but had no effect in mice lacking NKCC1. Hydrochlorothiazide, a blocker of the Na<jats:sup>+<\/jats:sup>\u2013Cl<jats:sup>\u2212<\/jats:sup> cotransporter, had only a small effect. DIDS, a blocker of some KCC cotransporters and Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> exchangers, reduced the EOG in epithelia from both wild\u2010type and <jats:italic>NKCC1<\/jats:italic> knockout mice. A combination of bumetanide and DIDS decreased the response more than either drug alone. However, no combination of drugs completely abolished the Cl<jats:sup>\u2212<\/jats:sup> component of the response. These results support the involvement of both NKCC1 and one or more DIDS\u2010sensitive transporters in Cl<jats:sup>\u2212<\/jats:sup> accumulation in olfactory receptor neurons.<\/jats:p>","DOI":"10.1113\/jphysiol.2007.129601","type":"journal-article","created":{"date-parts":[[2007,7,27]],"date-time":"2007-07-27T00:30:00Z","timestamp":1185496200000},"page":"1005-1020","source":"Crossref","is-referenced-by-count":66,"title":["Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium"],"prefix":"10.1113","volume":"583","author":[{"given":"William T.","family":"Nickell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy K.","family":"Kleene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven 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