{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:13:20Z","timestamp":1790838800629,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,5,4]],"date-time":"2007-05-04T00:00:00Z","timestamp":1178236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,5,15]]},"abstract":"<jats:p>The 5\u2010hydroxytryptamine (5\u2010HT) type 2 receptor family is involved in multiple physiological functions in smooth muscle including proliferation, differentiation and contraction. In myometrium, 5\u2010HT<jats:sub>2<\/jats:sub> receptors not only play a role in contraction but also regulate the activity of hypertrophic genes during pregnancy. Here we investigated whether 5\u2010HT<jats:sub>2A<\/jats:sub> receptors were up\u2010regulated during gestation and whether changes in expression could be correlated with changes in 5\u2010HT\u2010induced contractions in late pregnant myometrium. 5\u2010HT tension dose\u2013response curves showed that 5\u2010HT\u2010induced myometrial contractility is drastically increased in late pregnancy when compared to non\u2010pregnant conditions. The 5\u2010HT maximum tension (% of 80 m<jats:sc>m<\/jats:sc> KCl contracture) increased from 17 \u00b1 2% in non\u2010pregnant to 54 \u00b1 7% in late pregnant myometrium. This tension increase took place without significant changes in the 5\u2010HT sensitivity as EC<jats:sub>50<\/jats:sub> values were similar in non\u2010pregnant and late pregnant myometrium (0.11 \u00b1 0.03 \u03bc<jats:sc>m<\/jats:sc> and 0.17 \u00b1 0.02 \u03bc<jats:sc>m<\/jats:sc>, respectively). The increased 5\u2010HT\u2010induced contraction at the end of pregnancy was associated with up\u2010regulation of 5\u2010HT<jats:sub>2A<\/jats:sub> transcript (\u223c5\u2010fold) and protein (\u223c6\u2010fold) levels. These functional and biochemical studies provide evidence that myometrium remodelling during pregnancy is in part associated with up\u2010regulation of 5\u2010HT<jats:sub>2A<\/jats:sub> transcript and protein levels resulting in higher 5\u2010HT\u2010induced contractile responses. We conclude that the higher 5\u2010HT\u2010induced contractile response results from a higher density of 5\u2010HT<jats:sub>2A<\/jats:sub> receptors having the same properties as in non\u2010pregnant myometrium.<\/jats:p>","DOI":"10.1113\/jphysiol.2007.129726","type":"journal-article","created":{"date-parts":[[2007,3,30]],"date-time":"2007-03-30T00:35:00Z","timestamp":1175214900000},"page":"91-97","source":"Crossref","is-referenced-by-count":22,"title":["Increased 5\u2010HT contractile response in late pregnant rat myometrium is associated with a higher density of 5\u2010HT<sub>2A<\/sub> receptors"],"prefix":"10.1113","volume":"581","author":[{"given":"Tamara Y.","family":"Minosyan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansoureh","family":"Eghbali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligia","family":"Toro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Stefani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,4]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"860","article-title":"Effect of nitrendipine, diltiazem, trifluoperazine and pimozide on serotonin2 (5\u2010HT2) receptor activation in the rat uterus and jugular vein.","volume":"238","author":"Cohen ML","year":"1986","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_3_1","first-page":"770","article-title":"Role of 5\u2010HT2 receptors in serotonin\u2010induced contractions of nonvascular smooth muscle.","volume":"232","author":"Cohen ML","year":"1985","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/415175a"},{"key":"e_1_2_5_5_1","first-page":"1125","article-title":"Isolation and characterization of the rat 5\u2010hydroxytryptamine type 2 receptor promoter: constitutive and inducible activity in myometrial smooth muscle cells.","volume":"45","author":"Du YL","year":"1994","journal-title":"Mol Pharmacol"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2005.05.011"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(83)90545-9"},{"key":"e_1_2_5_8_1","first-page":"244","article-title":"Mechanisms of specific change by estradiol in sensitivity of rat uterus to serotonin.","volume":"229","author":"Ichida S","year":"1984","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod35.5.1131"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(94)90048-5"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.2.368"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0100129"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-002-0636-9"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-3623(92)90233-A"},{"key":"e_1_2_5_15_1","first-page":"256","article-title":"Ethodin: pharmacological evidence of the interaction between smooth muscle and mast cells in the myometrium.","volume":"282","author":"Rudolph MI","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1993.tb15178.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(93)90256-J"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205094200"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20040364"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200110000-00006"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1983.tb10059.x"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2007.129726","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2007.129726","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T13:35:16Z","timestamp":1697290516000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2007.129726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,4]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,5,15]]}},"alternative-id":["10.1113\/jphysiol.2007.129726"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2007.129726","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,4]]}}}