{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:20:22Z","timestamp":1791829222202,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,6,6]],"date-time":"2007-06-06T00:00:00Z","timestamp":1181088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,6,15]]},"abstract":"<jats:p>Rises in intracellular calcium are essential for contraction of human myometrial smooth muscle (HMSM) and hence parturition. The T\u2010type calcium channel may play a role in this process. The aim was to investigate the role of the T\u2010type calcium channel in HMSM by characterizing mRNA expression, protein localization, electrophysiological properties and function of the channel subunits Ca<jats:sub>v<\/jats:sub>3.1(\u03b11G), Ca<jats:sub>v<\/jats:sub>3.2(\u03b11H), and Ca<jats:sub>v<\/jats:sub>3.3(\u03b11I). QRT\u2010PCR, immunohistochemistry, electrophysiology and <jats:italic>invitro<\/jats:italic> contractility were performed on human myometrial samples from term, preterm, labour and not in labour. QRT\u2010PCR analysis of Ca<jats:sub>v<\/jats:sub>3.1, Ca<jats:sub>v<\/jats:sub>3.2 and Ca<jats:sub>v<\/jats:sub>3.3 demonstrated expression of Ca<jats:sub>v<\/jats:sub>3.1 and Ca<jats:sub>v<\/jats:sub>3.2 with no significant change (<jats:italic>P<\/jats:italic> &gt; 0.05) associated with gestation or labour status. Immunohistochemistry localized Ca<jats:sub>v<\/jats:sub>3.1 to myometrial and vascular smooth muscle cells whilst Ca<jats:sub>v<\/jats:sub>3.2 localized to vascular endothelial cells and invading leucocytes. Voltage clamp studies demonstrated a T\u2010type current in 55% of cells. Nickel block of T\u2010type current was voltage sensitive (IC<jats:sub>50<\/jats:sub> of 118.57 \u00b1 68.9 \u03bc<jats:sc>m<\/jats:sc> at \u221230 mV). Activation and inactivation curves of <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub> currents in cells expressing T\u2010type channels overlapped demonstrating steady state window currents at the resting membrane potential of myometrium at term. Current clamp analysis demonstrated that hyperpolarizing pulses to a membrane potential greater than \u221280 mV elicited rebound calcium spikes that were blocked reversibly by 100 \u03bc<jats:sc>m<\/jats:sc> nickel. Contractility studies demonstrated a reversible decrease in contraction frequency during application of 100 \u03bc<jats:sc>m<\/jats:sc> nickel (<jats:italic>P<\/jats:italic> &lt; 0.05). We conclude that the primary T\u2010type subunit expressed in some MSMCs is Ca<jats:sub>v<\/jats:sub>3.1. We found that application of 100 \u03bc<jats:sc>m<\/jats:sc> nickel to spontaneously contracting human myometrium reversibly slows contraction frequency.<\/jats:p>","DOI":"10.1113\/jphysiol.2007.132126","type":"journal-article","created":{"date-parts":[[2007,4,20]],"date-time":"2007-04-20T00:14:14Z","timestamp":1177028054000},"page":"915-926","source":"Crossref","is-referenced-by-count":55,"title":["Characterization of the molecular and electrophysiological properties of the T\u2010type calcium channel in human myometrium"],"prefix":"10.1113","volume":"581","author":[{"given":"Andrew M.","family":"Blanks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng\u2010Hang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anatoly","family":"Shmygol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Bru\u2010Mercier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirley","family":"Astle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Thornton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6,6]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.265.4.C976"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah158"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.21.5.514"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.1.103"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703352"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2006.04.027"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/4.5.673"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.56.6.1192"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1224-7"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1993.0002"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1721"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)00343-9"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00595685"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702685"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0769p.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-06-01912.1999"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)77134-1"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000090200"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000197851.11031.9c"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020688019792"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000089850"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000082333"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70390-X"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.229af.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(93)90513-I"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00018.2002"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1124\/mi.4.1.16"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/36110"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(97)90068-3"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.03.099"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(93)90006-5"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsgi.2005.02.007"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002329900059"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2007.132126","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2007.132126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T02:45:44Z","timestamp":1696992344000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2007.132126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,6]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6,15]]}},"alternative-id":["10.1113\/jphysiol.2007.132126"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2007.132126","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,6]]}}}