{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:58:15Z","timestamp":1791917895784,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,9,19]],"date-time":"2007-09-19T00:00:00Z","timestamp":1190160000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p>Electrical rhythmicity in the renal pelvis provides the fundamental drive for the peristaltic contractions that propel urine from the kidney to bladder for storage until micturition. Although atypical smooth muscles (ASMCs) within the most proximal regions of the renal pelvis have long been implicated as the pacemaker cells, the presence of a sparsely distributed population of rhythmically active<jats:italic>Kit<\/jats:italic>\u2010positive interstitial cells of Cajal\u2010like cells (ICC\u2010LCs) have confounded our understanding of pelviureteric peristalsis. We have recorded the electrical activity and separately visualized changes in intracellular Ca<jats:sup>2+<\/jats:sup>concentration in typical smooth muscle cells (TSMCs), ASMCs and ICC\u2010LCs using intracellular microelectrodes and a fluorescent Ca<jats:sup>2+<\/jats:sup>indicator, fluo\u20104. Nifedipine (1\u201310 \u03bc<jats:sc>m<\/jats:sc>)\u2010sensitive driven action potentials and Ca<jats:sup>2+<\/jats:sup>waves (frequency 6\u201315 min<jats:sup>\u22121<\/jats:sup>) propagated through the TSMC layer at a velocity of 1\u20132 mm s<jats:sup>\u22121<\/jats:sup>. High frequency (10\u201340 min<jats:sup>\u22121<\/jats:sup>) Ca<jats:sup>2+<\/jats:sup>transients and spontaneous transient depolarizations (STDs) were recorded in ASMCs in the absence or presence of 1 \u03bc<jats:sc>m<\/jats:sc>nifedipine. ICC\u2010LCs displayed low frequency (1\u20133 min<jats:sup>\u22121<\/jats:sup>) Ca<jats:sup>2+<\/jats:sup>transients which we speculated arose from cells that displayed action potentials with long plateaus (2\u20135 s). Neither electrical activity propagated over distances &gt; 50 \u03bcm. In 1 \u03bc<jats:sc>m<\/jats:sc>nifedipine, ASMCs or ICC\u2010LCs separated by &lt; 30 \u03bcm displayed some synchronicity in their Ca<jats:sup>2+<\/jats:sup>transient discharge suggesting that they may well be acting as \u2018point sources\u2019 of excitation to the TSMC layer. We speculate that ASMCs act as the primary pacemaker in the renal pelvis while ICC\u2010LCs play a supportive role, but can take over pacemaking in the absence of the proximal pacemaker drive.<\/jats:p>","DOI":"10.1113\/jphysiol.2007.137034","type":"journal-article","created":{"date-parts":[[2007,7,27]],"date-time":"2007-07-27T00:30:00Z","timestamp":1185496200000},"page":"1049-1068","source":"Crossref","is-referenced-by-count":61,"title":["Spontaneous electrical and Ca<sup>2+<\/sup>signals in typical and atypical smooth muscle cells and interstitial cell of Cajal\u2010like cells of mouse renal pelvis"],"prefix":"10.1113","volume":"583","author":[{"given":"Richard J.","family":"Lang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hikaru","family":"Hashitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary 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