{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:43:01Z","timestamp":1791272581442,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,9,28]],"date-time":"2007-09-28T00:00:00Z","timestamp":1190937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:p>Previous studies have shown that catecholamine secretion from the adrenal medulla plays a critical role in chronic intermittent hypoxia (CIH)\u2010induced alterations in cardiovascular function. In the present study we examined the cellular mechanisms associated with the effects of CIH on adrenal chromaffin cell catecholamine secretion. Experiments were performed on adult male mice (C57\/BL6) that were exposed to 1\u20134 days of CIH or to normoxia. Perforated patch electrical capacitance recordings were performed on freshly prepared adrenal medullary slices that permit separating the chromaffin cell secretion from sympathetic input. CIH resulted in a significant increase in the readily releasable pool (RRP) of secretory granules, and decreased stimulus\u2010evoked Ca<jats:sup>2+<\/jats:sup> influx. Continuous hypoxia (CH) either for 2.5 h (equivalent to hypoxic duration accumulated over 4 days of CIH) or for 4 days were ineffective in evoking changes in the RRP and Ca<jats:sup>2+<\/jats:sup> influx. CIH activated PKC in adrenal medullae as evidenced by increased phosphorylation of PKC at Thr<jats:sup>514<\/jats:sup> and PKC inhibitors prevented CIH\u2010induced increases in the RRP and restored stimulus\u2010evoked attenuation of Ca<jats:sup>2+<\/jats:sup> influx. CIH resulted in elevated thio\u2010barbituric acid reactive substances (TBARSs, an index of oxidized proteins) and an antioxidant prevented CIH\u2010induced changes in the RRP, suggesting the involvement of reactive oxygen species (ROS). These results demonstrate that CIH increases the RRP in adrenal chromaffin cells via ROS\u2010mediated activation of PKC and suggest that CIH can directly affect the secretory capacity of chromaffin cells and contribute, in part, to elevated catecholamine levels.<\/jats:p>","DOI":"10.1113\/jphysiol.2007.140624","type":"journal-article","created":{"date-parts":[[2007,8,17]],"date-time":"2007-08-17T00:25:25Z","timestamp":1187310325000},"page":"313-319","source":"Crossref","is-referenced-by-count":32,"title":["Increased secretory capacity of mouse adrenal chromaffin cells by chronic intermittent hypoxia: involvement of protein kinase C"],"prefix":"10.1113","volume":"584","author":[{"given":"Barbara A.","family":"Kuri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shakil A.","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyue\u2010An","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanduri R.","family":"Prabhakar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey B.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,9,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.83.1.95"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.01213.2004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.053918"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI16826"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000196732"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.34.2.309"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80147-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374600"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00277-6"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.21.11233"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.112524"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00613.2002"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5687(99)00113-9"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2005.04209.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02988.x"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00026-6"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-02-00589.1999"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80504-8"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80812-0"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8235626"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3048"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242624699"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2007.140624","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2007.140624","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T01:15:27Z","timestamp":1696986927000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2007.140624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,28]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1113\/jphysiol.2007.140624"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2007.140624","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9,28]]}}}