{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:19:43Z","timestamp":1792145983679,"version":"4.4.0"},"reference-count":55,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2008,7,31]],"date-time":"2008-07-31T00:00:00Z","timestamp":1217462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p>Fructose consumption has increased dramatically but little is known about mechanisms regulating the intestinal fructose transporter GLUT5 <jats:italic>in vivo<\/jats:italic>. In neonatal rats, GLUT5 can be induced only by luminal fructose and only after 14 days of age, unless the gut is primed with dexamethasone prior to fructose perfusion. To elucidate the mechanisms underlying dexamethasone modulation of GLUT5 development, we first identified the receptor mediating its effects then determined whether those effects were genomic. The glucocorticoid receptor (GR) antagonist RU486 dose\u2010dependently prevented the dexamethasone\u2010mediated effects on body weight, intestinal arginase2 (a known GR\u2010regulated gene) and GLUT5. In contrast, an antagonist of the mineralocorticoid receptor as well as agonists of progesterone (PR) and pregnane\u2010X (PXR) receptors did not block the effects of dexamethasone. These receptor antagonists and agonists had no effect on the intestinal glucose transporter SGLT1. Translocation of the GR into the enterocyte nucleus occurred only in dexamethasone\u2010injected pups perfused with fructose, was accompanied by marked increases in brush border GLUT5 abundance, and was blocked by RU486. A priming duration of \u223c24 h is optimal for induction but actinomycin D injection before dexamethasone priming prevented dexamethasone from allowing luminal fructose to induce GLUT5. Actinomycin D had no effect on dexamethasone\u2010independent fructose\u2010induced increases in glucose\u20106\u2010phosphatase mRNA abundance, suggesting that it did not prevent fructose\u2010induction of GLUT5, but instead prevented dexamethasone\u2010induced synthesis of an intermediate required by fructose for GLUT5 regulation. In suckling rats &lt; 14 days old, developmental regulation of transporters may involve cross\u2010talk between hormonal signals modulating intestinal maturation and nutrient signals regulating specific transporters.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.155226","type":"journal-article","created":{"date-parts":[[2008,6,13]],"date-time":"2008-06-13T01:54:19Z","timestamp":1213322059000},"page":"3657-3673","source":"Crossref","is-referenced-by-count":38,"title":["Developmental reprogramming of rat GLUT5 requires glucocorticoid receptor translocation to the nucleus"],"prefix":"10.1113","volume":"586","author":[{"given":"V\u00e9ronique","family":"Douard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye\u2010In","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Summer","family":"Elshenawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lagunoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo P.","family":"Ferraris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,7,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00454.2004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00319.2001"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(95)90210-4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.20.3195"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00252.2006"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00655.2006"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"14523","DOI":"10.1016\/S0021-9258(18)42067-4","article-title":"Fructose transporter in human spermatozoa and small intestine is GLUT5.","volume":"267","author":"Burant CF","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00550.2004"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00056.2004"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.10.12.1489"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0906"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"G822","DOI":"10.1152\/ajpgi.1990.259.5.G822","article-title":"Luminal glucose concentrations in the gut under normal conditions.","volume":"259","author":"Ferraris RP","year":"1990","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/129.4.799"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0305-0491(89)90002-3","article-title":"Brush border membrane sucrase\u2010isomaltase, maltase\u2010glucoamylase and trehalase in mammals. Comparative development, effects of glucocorticoids, molecular mechanisms, and phylogenetic implications","volume":"94","author":"Galand G","year":"1989","journal-title":"Comp Biochem Physiol B"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.049247"},{"key":"e_1_2_5_17_1","first-page":"362","article-title":"[The role of facilitated diffusion in glucose transport across the apical membrane of enterocytes].","volume":"92","author":"Gromova LV","year":"2006","journal-title":"Ross Fiziol Zh Im I M Sechenova"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/79.5.774"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(02)00589-8"},{"key":"e_1_2_5_20_1","volume-title":"Physiology of The Gastrointestinal Tract","author":"Henning S","year":"1987"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.04.070"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.040014"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(00)00357-X"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.3.G666"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.280.1.G113"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00689734"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2007.07.003"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/136.9.2308"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80900-9"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/014860719902300502"},{"key":"e_1_2_5_31_1","first-page":"1029","article-title":"Novel insights into mechanisms of glucocorticoid action and the development of new glucocorticoid receptor ligands.","volume":"73","author":"Lowenberg M","year":"2007","journal-title":"Steroids"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2006.04.005"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)04041-1"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00487.2002"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00011.2007"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00470.2003"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(82)90160-1"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.6.1781"},{"key":"e_1_2_5_39_1","first-page":"2","article-title":"U.S. per capita food supply trends: more calories, refined carbohydrates, and fats","volume":"25","author":"Putnam J","year":"2002","journal-title":"Food Rev"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0204068"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1677\/jme.0.0310573"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2004.04079.x"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(02)00263-7"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-0760(00)00125-4"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00531.2001"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-199802000-00025"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/09513590701267727"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200106000-00012"},{"key":"e_1_2_5_49_1","first-page":"839.3","article-title":"The effects of dephosphorylation signal on glucocorticoid hormone\u2010mediating GLUT5 expression in intestinal cells.","volume":"21","author":"Takabe S","year":"2007","journal-title":"FASEB J"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2006-0981"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.12.015"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.002315"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2006.01746.x"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(92)90635-3"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00412.2001"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1307"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2008.155226","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2008.155226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T16:06:53Z","timestamp":1697040413000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2008.155226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,31]]},"references-count":55,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["10.1113\/jphysiol.2008.155226"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2008.155226","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,31]]}}}