{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T20:12:24Z","timestamp":1790539944953,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2008,9,10]],"date-time":"2008-09-10T00:00:00Z","timestamp":1221004800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p>Mammalian K<jats:sup>+<\/jats:sup> homeostasis results from highly regulated renal and intestinal absorption and secretion, which balances the unregulated K<jats:sup>+<\/jats:sup> intake. Aldosterone is known to enhance both renal and colonic K<jats:sup>+<\/jats:sup> secretion. In mouse distal colon K<jats:sup>+<\/jats:sup> secretion occurs exclusively via luminal K<jats:sub>Ca<\/jats:sub>1.1 (BK) channels. Here we investigate if aldosterone stimulates colonic K<jats:sup>+<\/jats:sup> secretion via BK channels. Luminal Ba<jats:sup>2+<\/jats:sup> and iberiotoxin (IBTX)\u2010sensitive electrogenic K<jats:sup>+<\/jats:sup> secretion was measured in Ussing chambers. <jats:italic>In vivo<\/jats:italic> aldosterone was augmented via a high K<jats:sup>+<\/jats:sup> diet. High K<jats:sup>+<\/jats:sup> diet led to a 2\u2010fold increase of luminal Ba<jats:sup>2+<\/jats:sup> and IBTX\u2010sensitive short\u2010circuit current in distal mouse colonic mucosa. This effect was absent in BK \u03b1\u2010subunit\u2010deficient (BK<jats:sup>\u2212\/\u2212<\/jats:sup>) mice. The resting and diet\u2010induced K<jats:sup>+<\/jats:sup> secretion was stimulated by luminal ionomycin. In BK<jats:sup>\u2212\/\u2212<\/jats:sup> mice luminal ionomycin did not stimulate K<jats:sup>+<\/jats:sup> secretion. <jats:italic>In vitro<\/jats:italic> addition of aldosterone likewise triggered a 2\u2010fold increase in K<jats:sup>+<\/jats:sup> secretion, which was inhibited by the mineralocorticoid receptor antagonist spironolactone and the BK channel blocker IBTX. Semi\u2010quantification of mRNA from colonic crypts showed up\u2010regulation of BK \u03b1\u2010 and \u03b2<jats:sub>2<\/jats:sub>\u2010subunits in high K<jats:sup>+<\/jats:sup> diet mice. The BK channel could be detected luminally in colonic crypt cells by immunohistochemistry. The expression level of the channel in the luminal membrane was strongly up\u2010regulated in K<jats:sup>+<\/jats:sup>\u2010loaded animals. Taken together, these data strongly suggest that aldosterone\u2010induced K<jats:sup>+<\/jats:sup> secretion occurs via increased expression of luminal BK channels.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.156968","type":"journal-article","created":{"date-parts":[[2008,7,11]],"date-time":"2008-07-11T00:34:47Z","timestamp":1215736487000},"page":"4251-4264","source":"Crossref","is-referenced-by-count":78,"title":["Aldosterone increases K<sub>Ca<\/sub>1.1 (BK) channel\u2010mediated colonic K<sup>+<\/sup> secretion"],"prefix":"10.1113","volume":"586","author":[{"given":"Mads V.","family":"S\u00f8rensen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana 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