{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:55:25Z","timestamp":1791168925382,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2008,9,15]],"date-time":"2008-09-15T00:00:00Z","timestamp":1221436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2008,9,15]]},"abstract":"<jats:p>Visceral hypersensitivity is an important clinical feature associated with irritable bowel syndrome which in some patients has been linked to prior infection. Here we employ an animal model in which transient infection leads to persistent gut dysfunction to investigate the role of altered 5\u2010HT metabolism upon afferent mechanosensensitivity in the post\u2010infected gut. Jejunal segments isolated from <jats:italic>Trichinella spiralis<\/jats:italic>\u2010infected mice were used to assess 5\u2010HT metabolism whilst afferent activity in <jats:italic>T. spiralis<\/jats:italic>\u2010infected mice was studied by extracellular recordings from jejunal mesenteric afferent bundles and patch clamp recordings of isolated nodose ganglion neurons (NGNs). During acute infection, intestinal 5\u2010HT content and release increased, 5\u2010HT turnover decreased and afferent discharge in response to mechanical stimulation was attenuated. By day 28 post infection (PI), 5\u2010HT turnover had normalized, but 5\u2010HT content and release were still elevated. This was associated with afferent mechano\u2010hypersensitivity, which persisted for 8 weeks PI and was susceptible to 5\u2010HT<jats:sub>3<\/jats:sub> receptor blockade. NGNs from post\u2010infected animals were more excitable than controls but their current densities in response to 2\u2010methyl\u20105\u2010HT were lower. <jats:italic>T. spiralis<\/jats:italic> infection increased mucosal 5\u2010HT bioavailability and affected the spontaneous activity and mechanosensitivity of gastrointestinal sensory nerves. This involved an initial hyposensitivity occurring during acute infection followed by long\u2010term hypersensitivity in the post\u2010infectious period that was in part mediated by 5\u2010HT acting via 5\u2010HT<jats:sub>3<\/jats:sub> receptors. Functional down\u2010regulation of 5\u2010HT<jats:sub>3<\/jats:sub> receptors also occurs in the post\u2010infected animals, which may represent an adaptive response to increased mucosal 5\u2010HT bioavailability.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.156984","type":"journal-article","created":{"date-parts":[[2008,7,25]],"date-time":"2008-07-25T00:44:48Z","timestamp":1216946688000},"page":"4517-4530","source":"Crossref","is-referenced-by-count":76,"title":["Afferent hypersensitivity in a mouse model of post\u2010inflammatory gut dysfunction: role of altered serotonin 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