{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:28:27Z","timestamp":1785774507670,"version":"3.56.0"},"reference-count":57,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,5,14]],"date-time":"2009-05-14T00:00:00Z","timestamp":1242259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2009,5,15]]},"abstract":"<jats:p>Pancreatic duct epithelial cells (PDECs) have been shown to express calcium activated chloride channels (CaCCs) and there is evidence for their involvement in fluid secretion from these cells. The molecular identity of the CaCC in PDECs remains unknown. Recently, the bestrophin family of proteins have been proposed as a potential molecular candidate for CaCCs. Expression of bestrophins is strongly correlated with the function of CaCCs in a variety of tissues. In the present study, the expression of bestrophins has been investigated in the cystic fibrosis pancreatic duct cell line, CFPAC\u20101. Iodide efflux analysis was used to characterise native CaCCs in CFPAC\u20101 cell monolayers. Efflux was induced with the addition of UTP (100 \u03bc<jats:sc>m<\/jats:sc>, 10.2 \u00b1 1.5 nmol min<jats:sup>\u22121<\/jats:sup>), which was blocked by the chloride channel blockers niflumic acid (81%) and DIDS (90%). The UTP\u2010stimulated iodide efflux was shown to be Ca<jats:sup>2+<\/jats:sup> dependent and cAMP independent. RT\u2010PCR analysis of RNA isolated from CFPAC\u20101 cells demonstrated positive identification of all four human bestrophin mRNAs. Western blot of CFPAC\u20101 cell protein isolates with antibodies specific to human bestrophin 1 (hBest1) showed that hBest1 protein was expressed in this cell line. HBest1 was present on the cell surface, demonstrated using biotinylation and confocal imaging, as well as in the cytoplasm. SiRNA\u2010mediated silencing of hBest1 in CFPAC\u20101 cells reduced the UTP\u2010stimulated iodide efflux by around 40%. This study provides evidence that the bestrophins are expressed in pancreatic duct cells and, more specifically, that hBest1 plays a role in the CaCCs found in these cells.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.159087","type":"journal-article","created":{"date-parts":[[2009,2,24]],"date-time":"2009-02-24T01:24:25Z","timestamp":1235438665000},"page":"2211-2224","source":"Crossref","is-referenced-by-count":19,"title":["Bestrophin expression and function in the human pancreatic duct cell line, CFPAC\u20101"],"prefix":"10.1113","volume":"587","author":[{"given":"Laura L.","family":"Marsey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Winpenny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,5,14]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"1473","volume-title":"Physiology of the Gastrointestinal 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