{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:37:12Z","timestamp":1791214632218,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2008,11,19]],"date-time":"2008-11-19T00:00:00Z","timestamp":1227052800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2008,11,15]]},"abstract":"<jats:p>Muscle regeneration following injury is dependent on the ability of muscle satellite cells to activate, proliferate and fuse with damaged fibres. This process is controlled by the myogenic regulatory factors (MRF). Little is known about the temporal relation of the MRF with the expression of known myogenic growth factors (i.e. IGF\u20101) in humans following muscle damage. Eight subjects (20.6 \u00b1 2.1 years; 81.4 \u00b1 9.8 kg) performed 300 lengthening contractions (180 deg s<jats:sup>\u22121<\/jats:sup>) of their knee extensors in one leg on a dynamometer. Blood and muscle samples were collected before and at 4 (T4), 24 (T24), 72 (T72) and 120 h (T120) post\u2010exercise. <jats:italic>Mechano growth factor (MGF)<\/jats:italic>, <jats:italic>IGF\u20101Ea<\/jats:italic> and <jats:italic>IGF\u20101Eb<\/jats:italic> mRNA were quantified. Serum IGF\u20101 did not change over the post\u2010exercise time course. <jats:italic>IGF\u20101Ea<\/jats:italic> and <jats:italic>IGF\u20101Eb<\/jats:italic> mRNA increased \u223c4\u2010 to 6\u2010fold by T72 (<jats:italic>P<\/jats:italic> &lt; 0.01) and <jats:italic>MGF<\/jats:italic> mRNA expression peaked at T24 (<jats:italic>P<\/jats:italic>= 0.005). <jats:italic>MyoD<\/jats:italic> mRNA expression increased \u223c2\u2010fold at T4 (<jats:italic>P<\/jats:italic> &lt; 0.05). <jats:italic>Myf5<\/jats:italic> expression peaked at T24 (<jats:italic>P<\/jats:italic> &lt; 0.05), while <jats:italic>MRF4<\/jats:italic> and <jats:italic>myogenin<\/jats:italic> mRNA expression peaked at T72 (<jats:italic>P<\/jats:italic> &lt; 0.05). <jats:italic>Myf5<\/jats:italic> expression strongly correlated with the increase in <jats:italic>MGF<\/jats:italic> mRNA (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.83; <jats:italic>P<\/jats:italic>= 0.03), while <jats:italic>MRF4<\/jats:italic> was correlated with both <jats:italic>IGF\u20101Ea<\/jats:italic> and <jats:italic>\u2010Eb<\/jats:italic> (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.90; <jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>= 0.81, respectively; <jats:italic>P<\/jats:italic> &lt; 0.05). Immunofluorescence analysis showed IGF\u20101 protein expression localized to satellite cells at T24, and to satellite cells and the myofibre at T72 and T120; IGF\u20101 was not detected at T0 or T4. These results suggest that the temporal response of <jats:italic>MGF<\/jats:italic> is probably related to the activation\/proliferation phase of the myogenic programme as marked by an increase in both <jats:italic>Myf5<\/jats:italic> and <jats:italic>MyoD<\/jats:italic>, while <jats:italic>IGF\u20101Ea<\/jats:italic> and \u2010<jats:italic>Eb<\/jats:italic> may be temporally related to differentiation as marked by an increase in <jats:italic>MRF4<\/jats:italic> and <jats:italic>myogenin<\/jats:italic> expression following acute muscle damage.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.160176","type":"journal-article","created":{"date-parts":[[2008,9,26]],"date-time":"2008-09-26T00:24:44Z","timestamp":1222388684000},"page":"5549-5560","source":"Crossref","is-referenced-by-count":143,"title":["Co\u2010expression of IGF\u20101 family members with myogenic regulatory factors following acute damaging muscle\u2010lengthening contractions in 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