{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:30:30Z","timestamp":1792805430294,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,14]],"date-time":"2009-01-14T00:00:00Z","timestamp":1231891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2009,1,15]]},"abstract":"<jats:p>The coupling between Ca<jats:sup>2+<\/jats:sup> extrusion and H<jats:sup>+<\/jats:sup> uptake by the ubiquitous plasma membrane calcium ATPase (PMCA) has not been measured in any neurone. I have investigated this with Ca<jats:sup>2+<\/jats:sup>\u2010 and pH\u2010sensitive microelectrodes in large voltage\u2010clamped snail neurones, which have no Na<jats:sup>+<\/jats:sup>\u2013Ca<jats:sup>2+<\/jats:sup> exchangers. The recovery of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> and surface pH after a brief depolarization or Ca<jats:sup>2+<\/jats:sup> injection was not slowed by hyperpolarization to \u221290 mV from a holding potential of \u221250 mV, consistent with a 1 Ca<jats:sup>2+<\/jats:sup> : 2 H<jats:sup>+<\/jats:sup> coupling ratio. Since Ca<jats:sup>2+<\/jats:sup> injections proved difficult to quantify, and Ca<jats:sup>2+<\/jats:sup> currents through channels were obscured by K<jats:sup>+<\/jats:sup> currents, Ba<jats:sup>2+<\/jats:sup> was used as a substitute. When the cell was bathed in Ca<jats:sup>2+<\/jats:sup>\u2010free Ba<jats:sup>2+<\/jats:sup> Ringer solution, the K<jats:sup>+<\/jats:sup> currents were blocked and large inward currents were revealed on depolarization. The Ca<jats:sup>2+<\/jats:sup>\u2010sensitive microelectrodes were sensitive to intracellular Ba<jats:sup>2+<\/jats:sup> as well as Ca<jats:sup>2+<\/jats:sup>. With equal depolarizations Ba<jats:sup>2+<\/jats:sup> entry appeared larger than Ca<jats:sup>2+<\/jats:sup> entry and generated similar but slower pH changes. Ba<jats:sup>2+<\/jats:sup> extrusion was insensitive to hyperpolarization, blocked by eosin or high pH, and about 5 times slower than Ca<jats:sup>2+<\/jats:sup> extrusion. The ratio of the pH change caused by the extrusion of unit charge of Ba<jats:sup>2+<\/jats:sup> influx to that caused by unit charge of H<jats:sup>+<\/jats:sup> injection was 0.85 \u00b1 0.08 (<jats:sc>s.e.m.<\/jats:sc>, <jats:italic>n<\/jats:italic>= 8), corresponding to a Ba<jats:sup>2+<\/jats:sup> : H<jats:sup>+<\/jats:sup> ratio of 1 : 1.7. Both this ratio and the electroneutrality of the PMCA suggest that the Ca<jats:sup>2+<\/jats:sup> : H<jats:sup>+<\/jats:sup> ratio is 1 : 2, ensuring that after a Ca<jats:sup>2+<\/jats:sup> influx [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> recovery is not influenced by the membrane potential and maximizes the conversion of Ca<jats:sup>2+<\/jats:sup> influxes into possible pH signals.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.162453","type":"journal-article","created":{"date-parts":[[2008,12,9]],"date-time":"2008-12-09T01:25:04Z","timestamp":1228785904000},"page":"315-327","source":"Crossref","is-referenced-by-count":67,"title":["The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H<sup>+<\/sup> for each Ca<sup>2+<\/sup> or Ba<sup>2+<\/sup> ion extruded"],"prefix":"10.1113","volume":"587","author":[{"given":"Roger C.","family":"Thomas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00583469"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.80.5.663"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019316"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00010.2003"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.2741\/2414"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.02.026"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1976.tb00344.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0079-6107(84)90009-9"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1976.sp011225"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(88)90029-2"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"C1577","DOI":"10.1152\/ajpcell.1993.264.6.C1577","article-title":"Inhibition of the red blood cell calcium pump by eosin and other fluorescein analogues","volume":"264","author":"Gatto C","year":"1993","journal-title":"Am J Physiol Cell Physiol"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00261a049"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2005.06.032"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"14268","DOI":"10.1016\/S0021-9258(17)36784-4","article-title":"Ca2+\/H+ countertransport and electrogenicity in proteoliposomes containing erythrocyte plasma membrane Ca\u2010ATPase and exogenous lipids","volume":"269","author":"Hao L","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020684"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(96)79778-3"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresrev.2004.11.004"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1980.sp013070"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200001170-00007"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1990.258.3.C552"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2007.12.005"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1016\/S0021-9258(18)34929-9","article-title":"The purified Ca2+ pump of human erythrocyte membranes catalyzes an electroneutral Ca2+\u2010H+ exchange in reconstituted liposomal systems","volume":"257","author":"Niggli V","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_5_24_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0006-3002(62)90708-4","article-title":"Studies on bacterial photophosphorylation. 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