{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:45:25Z","timestamp":1791276325619,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,6,12]],"date-time":"2009-06-12T00:00:00Z","timestamp":1244764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2009,6,15]]},"abstract":"<jats:p>Mouse parotid acinar cells express P2X<jats:sub>4<\/jats:sub> and P2X<jats:sub>7<\/jats:sub> receptors (mP2X<jats:sub>4<\/jats:sub>R and mP2X<jats:sub>7<\/jats:sub>R) whose physiological function remains undetermined. Here we show that mP2X<jats:sub>4<\/jats:sub>R expressed in HEK\u2010293 cells do not allow the passage of tetraethylammonium (TEA<jats:sup>+<\/jats:sup>) and promote little, if any, ethidium bromide (EtBr) uptake when stimulated with ATP or BzATP. In contrast, mP2X<jats:sub>7<\/jats:sub>R generates slowly decaying TEA<jats:sup>+<\/jats:sup> current, sustained Na<jats:sup>+<\/jats:sup> current and promotes robust EtBr uptake. However, ATP\u2010activated TEA<jats:sup>+<\/jats:sup> current from acinar cells was unlike that generated by mP2X<jats:sub>7<\/jats:sub>R or mP2X<jats:sub>4<\/jats:sub>R. Functional interactions between mP2X<jats:sub>4<\/jats:sub>R and mP2X<jats:sub>7<\/jats:sub>R were investigated in HEK cells co\u2010transfected with different mP2X<jats:sub>4<\/jats:sub> : mP2X<jats:sub>7<\/jats:sub> cDNA ratios and using solutions containing either TEA<jats:sup>+<\/jats:sup> or Na<jats:sup>+<\/jats:sup> ions. Co\u2010expressed channels generated a TEA<jats:sup>+<\/jats:sup> current that displayed faster decay during ATP stimulation than mP2X<jats:sub>7<\/jats:sub>R alone. Moreover, cells transfected with a 2 : 1 cDNA ratio displayed decaying kinetics similar to those observed in acinar cells. Concentration\u2013response curves in Na<jats:sup>+<\/jats:sup>\u2010containing solutions were constructed for heterologously expressed mP2X<jats:sub>4<\/jats:sub>R, mP2X<jats:sub>7<\/jats:sub>R and mP2X<jats:sub>4<\/jats:sub>R:mP2X<jats:sub>7<\/jats:sub>R co\u2010expressions as well as acinar cells. The EC<jats:sub>50<\/jats:sub> values determined were 11, 220, 434 and 442 \u03bc<jats:sc>m<\/jats:sc>, respectively. Na<jats:sup>+<\/jats:sup> currents generated by expressing mP2X<jats:sub>4<\/jats:sub>R or mP2X<jats:sub>7<\/jats:sub>R alone were potentiated by ivermectin (IVM). In contrast, IVM potentiation in acinar cells and HEK cells co\u2010expressing P2X<jats:sub>4<\/jats:sub> and P2X<jats:sub>7<\/jats:sub> (1 : 1 or 2 : 1 cDNA ratios) was seen only when the ATP concentration was lowered from 5 to 0.03 m<jats:sc>m<\/jats:sc>. Taken together our observations indicate a functional interaction between murine P2X<jats:sub>7<\/jats:sub> and P2X<jats:sub>4<\/jats:sub> receptors. Such interaction might occur in acinar cells to shape the response to extracellular ATP in salivary epithelia.<\/jats:p>","DOI":"10.1113\/jphysiol.2008.167395","type":"journal-article","created":{"date-parts":[[2009,4,30]],"date-time":"2009-04-30T00:34:06Z","timestamp":1241051646000},"page":"2887-2901","source":"Crossref","is-referenced-by-count":55,"title":["Functional interactions between P2X<sub>4<\/sub> and P2X<sub>7<\/sub> receptors from mouse salivary epithelia"],"prefix":"10.1113","volume":"587","author":[{"given":"Griselda","family":"Casas\u2010Pruneda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan 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