{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:03:00Z","timestamp":1791266580170,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,6,12]],"date-time":"2009-06-12T00:00:00Z","timestamp":1244764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2009,6,15]]},"abstract":"<jats:p>\n                    Carotid body (CB) glomus cells from rat express a TASK\u2010like background K\n                    <jats:sup>+<\/jats:sup>\n                    channel that is believed to play a critical role in the regulation of excitability and hypoxia\u2010induced increase in respiration. Here we studied the kinetic behaviour of single channel openings from rat CB cells to determine the molecular identity of the \u2018TASK\u2010like\u2019 K\n                    <jats:sup>+<\/jats:sup>\n                    channels. In outside\u2010out patches, the TASK\u2010like background K\n                    <jats:sup>+<\/jats:sup>\n                    channel in CB cells was inhibited &gt;90% by a reduction of pH\n                    <jats:sub>o<\/jats:sub>\n                    from 7.3 to 5.8. In cell\u2010attached patches with 140 m\n                    <jats:sc>m<\/jats:sc>\n                    KCl and 1 m\n                    <jats:sc>m<\/jats:sc>\n                    Mg\n                    <jats:sup>2+<\/jats:sup>\n                    in the bath and pipette solutions, two main open levels with conductance levels of \u223c14 pS and \u223c32 pS were recorded at a membrane potential of \u221260 mV. The K\n                    <jats:sup>+<\/jats:sup>\n                    channels showed kinetic properties similar to TASK\u20101 (\u223c14 pS), TASK\u20103 (\u223c32 pS) and TASK\u20101\/3 heteromer (\u223c32 pS). The presence of three TASK isoforms was tested by reducing [Mg\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    to \u223c0 m\n                    <jats:sc>m<\/jats:sc>\n                    , which had no effect on the conductance of TASK\u20101, but increased those of TASK\u20101\/3 and TASK\u20103 to 42 pS and 74 pS, respectively. In CB cells, the reduction of [Mg\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    to \u223c0 m\n                    <jats:sc>m<\/jats:sc>\n                    also caused the appearance of \u223c42 pS (TASK\u20101\/3\u2010like) and \u223c74 pS (TASK\u20103\u2010like) channels, in addition to the \u223c14 pS (TASK\u20101\u2010like) channel. The 42 pS channel was the most abundant, contributing \u223c75% of the current produced by TASK\u2010like channels. Ruthenium red (5 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    ) had no effect on TASK\u20101 and TASK\u20101\/3, but inhibited TASK\u20103 by 87%. In CB cells, ruthenium red caused \u223c12% inhibition of TASK\u2010like activity. Methanandamide reduced the activity of all three TASKs by 80\u201390%, and that of TASK\u2010like channels in CB cell also by \u223c80%. In CB cells, hypoxia caused inhibition of TASK\u2010like channels, including TASK\u20101\/3\u2010like channels. These results show that TASK\u20101, TASK\u20101\/3 and TASK\u20103 are all functionally expressed in isolated CB cells, and that the TASK\u20101\/3 heteromer provides the major part of the oxygen\u2010sensitive TASK\u2010like background K\n                    <jats:sup>+<\/jats:sup>\n                    conductance.\n                  <\/jats:p>","DOI":"10.1113\/jphysiol.2009.171181","type":"journal-article","created":{"date-parts":[[2009,4,29]],"date-time":"2009-04-29T20:34:06Z","timestamp":1241037246000},"page":"2963-2975","source":"Crossref","is-referenced-by-count":151,"title":["Heteromeric TASK\u20101\/TASK\u20103 is the major oxygen\u2010sensitive background K\n                    <sup>+<\/sup>\n                    channel in rat carotid body glomus cells"],"prefix":"10.1113","volume":"587","author":[{"given":"Donghee","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Cavanaugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Insook","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John L.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1408-04.2004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35051086"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1997.sp021890"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5687(99)00015-8"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2007.02.013"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00135.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00989-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107138200"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.63.3.646"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5687(99)00021-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.17.5464"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.100.3.401"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.032094"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.017590"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.081059"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.054387"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.01.137"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612054546824"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-31311-7_7"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.277.5.H1669"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.13.9340"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00276-5"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5310"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013888"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00056408"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.1.47"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4254-07.2007"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.106898"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.83.3.1150"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.01.00204001"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2006.10.010"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2005.031922"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh1701.095632"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M200502200"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-19-07491.2001"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1810-08.2008"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.93.5.979"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707436"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00010.2003"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-9280-2_15"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-2572-1_18"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020606"},{"key":"e_1_2_5_44_1","first-page":"799","article-title":"Expression of inwardly rectifying K+ channels in the carotid body of rat","volume":"23","author":"Yamamoto Y","year":"2008","journal-title":"Histol Histopathol"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03181-5"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.5A6755.2005"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2009.171181","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2009.171181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T13:09:53Z","timestamp":1697029793000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2009.171181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,12]]},"references-count":45,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2009,6,15]]}},"alternative-id":["10.1113\/jphysiol.2009.171181"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2009.171181","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1160838.622254","asserted-by":"object"}]},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,12]]}}}