{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T21:16:39Z","timestamp":1771103799154,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,4,30]],"date-time":"2010-04-30T00:00:00Z","timestamp":1272585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:p>A delayed rectifier voltage\u2010gated K<jats:sup>+<\/jats:sup> channel (Kv) represents the largest ionic conductance of platelets and megakaryocytes, but is undefined at the molecular level. Quantitative RT\u2010PCR of all known Kv \u03b1 and ancillary subunits showed that only Kv1.3 (<jats:italic>KCNA3<\/jats:italic>) is substantially expressed in human platelets. Furthermore, megakaryocytes from Kv1.3<jats:sup>\u2212\/\u2212<\/jats:sup> mice or from wild\u2010type mice exposed to the Kv1.3 blocker margatoxin completely lacked Kv currents and displayed substantially depolarised resting membrane potentials. In human platelets, margatoxin reduced the P2X<jats:sub>1<\/jats:sub>\u2010 and thromboxaneA<jats:sub>2<\/jats:sub> receptor\u2010evoked [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> increases and delayed the onset of store\u2010operated Ca<jats:sup>2+<\/jats:sup> influx. Megakaryocyte development was normal in Kv1.3<jats:sup>\u2212\/\u2212<\/jats:sup> mice, but the platelet count was increased, consistent with a role of Kv1.3 in apoptosis or decreased platelet activation. We conclude that Kv1.3 forms the Kv channel of the platelet and megakaryocyte, which sets the resting membrane potential, regulates agonist\u2010evoked Ca<jats:sup>2+<\/jats:sup> increases and influences circulating platelet numbers.<\/jats:p>","DOI":"10.1113\/jphysiol.2010.188136","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T01:38:44Z","timestamp":1269308324000},"page":"1399-1406","source":"Crossref","is-referenced-by-count":46,"title":["Kv1.3 is the exclusive voltage\u2010gated K<sup>+<\/sup> channel of platelets and megakaryocytes: roles in membrane potential, Ca<sup>2+<\/sup> signalling and platelet count"],"prefix":"10.1113","volume":"588","author":[{"given":"Conor","family":"McCloskey","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Amisten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger T.","family":"Snowden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard K.","family":"Kaczmarek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Erlinge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison H.","family":"Goodall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian D.","family":"Forsythe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martyn P.","family":"Mahaut\u2010Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,4,30]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1055\/s-0037-1616102","article-title":"Serial determinations of platelet counts in mice by flow cytometry","volume":"86","author":"Alugupalli KR","year":"2001","journal-title":"Thromb Haemost"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2007.08.014"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00816.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2004.03.010"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.167387"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00844-4"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.38.22406"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46707-X"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"20971","DOI":"10.1016\/S0021-9258(19)36784-5","article-title":"The Shaw\u2010related potassium channel gene, Kv3.1, on human chromosome 11, encodes the type l K+ channel in T cells","volume":"267","author":"Grissmer S","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32312"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.10"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1182\/blood.V86.3.1043.1043","article-title":"Suppression of the delayed rectifier type of voltage gated K+ outward current in megakaryocytes from patients with myelogenous leukemias","volume":"86","author":"Kapural L","year":"1995","journal-title":"Blood"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018326"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304879200"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.7.1167"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.13.040195.003203"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020644"},{"key":"e_1_2_5_19_1","first-page":"277","article-title":"Patch\u2010clamp recordings of electrophysiological events in the platelet and megakaryocyte","volume":"273","author":"Mahaut\u2010Smith MP","year":"2004","journal-title":"Methods Mol Biol"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(03)75070-X"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016750"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.037"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for quantification in real\u2010time RT\u2010PCR","volume":"29","author":"Pfaffl M","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728220903018957"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0037-1615684"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.5.C1525"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2755"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804236105"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-02-0725"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03455.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308450100"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2010.188136","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2010.188136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T19:34:05Z","timestamp":1697312045000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2010.188136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,30]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1113\/jphysiol.2010.188136"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2010.188136","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,30]]}}}