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These reactions depend on a histidine residue on the channel that binds metal ions. If we prevent this histidine from binding metals, N<jats:sub>2<\/jats:sub>O cannot inhibit T\u2010currents. Mice that are treated with EUK\u2010134 to remove free radicals show little pain relief after N<jats:sub>2<\/jats:sub>O administration. This report provides new information on how N<jats:sub>2<\/jats:sub>O interacts with ion channels, helping our understanding of how this popular pain reliever works.<\/jats:p>","DOI":"10.1113\/jphysiol.2010.196220","type":"journal-article","created":{"date-parts":[[2010,11,9]],"date-time":"2010-11-09T06:11:30Z","timestamp":1289283090000},"page":"135-148","source":"Crossref","is-referenced-by-count":38,"title":["Free radical signalling underlies inhibition of Ca<sub>V<\/sub>3.2 T\u2010type calcium channels by nitrous oxide in the pain pathway"],"prefix":"10.1113","volume":"589","author":[{"given":"Peihan","family":"Orestes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damir","family":"Bojadzic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JeongHan","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Leach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Salajegheh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"DiGruccio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Nelson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Slobodan M.","family":"Todorovic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/S0022-3565(25)38223-6","article-title":"Adrenochrome reaction implicates oxygen radicals in metabolism of cyclosporine A and FK\u2010506 in rat and human liver microsomes","volume":"265","author":"Ahmed SS","year":"1993","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)11442-Y"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90343-E"},{"key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0022-3565(24)37225-8","article-title":"Synthetic combined superoxide dismutase\/catalase mimetics are protective as delayed treatment in a rat stroke model: a key role for reactive oxygen species in ischemic brain injury","volume":"284","author":"Baker K","year":"1998","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/j19680002886"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.09.002"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(80)90060-X"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600515"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/73288"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2307\/3574392"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.132274"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-183X.2006.00268.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/mi.4.5.3"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp004613"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.105.6.837"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1056-8719(00)00089-7"},{"key":"e_1_2_5_18_1","unstructured":"FreitasRA(1999).Nanomedicine. 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