{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T22:33:01Z","timestamp":1772922781721,"version":"3.50.1"},"reference-count":74,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,3,30]],"date-time":"2011-03-30T00:00:00Z","timestamp":1301443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p><jats:bold>Non\u2010technical summary\u2002<\/jats:bold> Most cellular processes are exquisitely sensitive to pH. Consequently our cells have a range of processes directed to control cellular pH. Plasma membrane transport proteins move acid or base across the plasma membrane to regulate pH precisely. We studied AE1 (also called Band 3) of erythrocytes and kidney cells, which rapidly transports the base, bicarbonate. AE1's high transport rate, combined with the surprisingly slow rates of H<jats:sup>+<\/jats:sup> diffusion in cytosol, led us to wonder whether AE1 changes the pH of its local environment. The key findings were that H<jats:sup>+<\/jats:sup> diffusion through the cytosol occurs at 0.6 \u03bcm s<jats:sup>\u22121<\/jats:sup>, and along the inner surface of the plasma membrane at only 0.01 \u03bcm s<jats:sup>\u22121<\/jats:sup>. We estimated that the size of the region of altered pH (H<jats:sup>+<\/jats:sup> microdomain) around AE1 is 0.3 \u03bcm in diameter. pH\u2010regulatory transporters, like AE1, have differential effects on their immediate environment, with implications for the regulation of nearby pH\u2010sensitive proteins.<\/jats:p>","DOI":"10.1113\/jphysiol.2010.201483","type":"journal-article","created":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T05:18:49Z","timestamp":1297142329000},"page":"1551-1569","source":"Crossref","is-referenced-by-count":33,"title":["Cytosolic H<sup>+<\/sup> microdomain developed around AE1 during AE1\u2010mediated Cl<sup>\u2212<\/sup>\/HCO<sub>3<\/sub><sup>\u2212<\/sup> exchange"],"prefix":"10.1113","volume":"589","author":[{"given":"Danielle E.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph R.","family":"Casey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,3,30]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"171","article-title":"Regulation of Na+\u2010independent Cl\u2212\/HCO3\n                  \u2212 exchangers by pH","volume":"2","author":"Alper SL","year":"2001","journal-title":"JOP"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00449.2006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.100925"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802134200"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0370961"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2010.06.020"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2824"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409741102"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.15.8521"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2820"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98470-X"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.6.C1111"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(97)00090-4"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081634"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"3626","DOI":"10.1016\/S0021-9258(18)61399-7","article-title":"Estimation of the number and turnover rate of Na+\/H+ exchangers in lymphocytes. Effect of phorbol ester and osmotic shrinking","volume":"262","author":"Dixon SJ","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00789a030"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(00)76293-X"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-36-1-59"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(78)90424-8"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1067-2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi026609p"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(75)85812-7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"9002","DOI":"10.1016\/S0021-9258(19)84480-0","article-title":"Monoclonal antibodies against human erythrocyte band 3 protein. Localization of proteolytic cleavage sites and stilbenedisulfonate\u2010binding lysine residues","volume":"261","author":"Jennings ML","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.2004.01361.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.019042"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470508169.ch20"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00375054"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702139200"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.115.5.599"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.06927.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-700-6_17"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(86)90009-2"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.10.5430"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1139\/y06-065"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(99)00022-2"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081847"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/28190"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704844200"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R800058200"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm002"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20091515"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2009.02.009"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8278814"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.2000.0353"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M403099200"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00166.2006"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.1.37"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01671.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.06.020"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.278.4.H1371"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013268"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3440221"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1139\/o02-152"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105959200"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.209aa.x"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.145185"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.2307\/1542286"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.051391"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.096560"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.197392"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801330200"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.35.22545"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00578a012"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00008.2008"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)99819-4"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013267"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2005.06.004"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0015111"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80408-4"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05876.x"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414355200"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009090921"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152066"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069982"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2010.201483","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2010.201483","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T22:01:39Z","timestamp":1698789699000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2010.201483"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,30]]},"references-count":74,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1113\/jphysiol.2010.201483"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2010.201483","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,30]]}}}