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The details of this process in astrocytes are not well understood, so we used a fluorescently labelled vesicle protein, synapto\u2010pHluorin (spH), to track how these fusions occurred. When astrocytes were mechanically stimulated we saw a slow burst of fusions, while other stimuli caused a relatively even sustained rate of fusion. We observed two distinct types of events, transient and full fusions, the proportion of which was stimulus dependent. Similarly, stability of the vesicle fusion pore with the plasma membrane varied with the stimulus. We describe the effects on fusion events resulting from expressing variants of exocytotic proteins, synaptotagmin 1 and SNAP25B. Studying the characteristics of astrocytic exocytosis will aid in the general understanding of this process and also events at the tripartite synapse, both in health and disease.<\/jats:p>","DOI":"10.1113\/jphysiol.2011.210435","type":"journal-article","created":{"date-parts":[[2011,7,12]],"date-time":"2011-07-12T04:59:26Z","timestamp":1310446766000},"page":"4271-4300","source":"Crossref","is-referenced-by-count":60,"title":["Temporal characteristics of vesicular fusion in astrocytes: examination of synaptobrevin 2\u2010laden vesicles at single vesicle resolution"],"prefix":"10.1113","volume":"589","author":[{"given":"Erik 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