{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:21:55Z","timestamp":1790072515152,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2011,12,14]],"date-time":"2011-12-14T00:00:00Z","timestamp":1323820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2011,12,15]]},"abstract":"<jats:p><jats:bold>Non\u2010technical summary\u2002<\/jats:bold> Dendritic spines of central neurons contain calcium stores, but their involvement in synaptic plasticity is not entirely clear. Synaptopodin, an actin\u2010binding molecule, has been localized in the neck of some dendritic spines, and assumed to play a role in regulation of calcium stores in the spines. In the present study we used flash photolysis of calcium inside dendritic spines of cultured hippocampal neurons loaded with the calcium sensor Fluo\u20104. An ultrafast UV flash generated a rapid rise in [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> followed by a monoexponential decay back to control level. In spines where synaptopodin was found in <jats:italic>post hoc<\/jats:italic> immunocytochemical analysis, the decay of elevated calcium was much prolonged compared to synaptopodin\u2010negative spines. This prolongation was eliminated by blockade of release of calcium from stores. Our results provide the first direct demonstration of calcium release from stores in dendritic spines of central neurons.<\/jats:p>","DOI":"10.1113\/jphysiol.2011.217315","type":"journal-article","created":{"date-parts":[[2011,10,25]],"date-time":"2011-10-25T04:41:30Z","timestamp":1319517690000},"page":"5987-5995","source":"Crossref","is-referenced-by-count":62,"title":["Synaptopodin regulates release of calcium from stores in dendritic spines of cultured hippocampal neurons"],"prefix":"10.1113","volume":"589","author":[{"given":"Eduard","family":"Korkotian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menahem","family":"Segal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1400"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20539"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00217893"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1832384100"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(20000306)418:2<164::AID-CNE4>3.0.CO;2-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80683-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)91322-7"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/78791"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(92)90551-H"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2964-05.2006"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.21.12068"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2006.11.006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1523\/JNEUROSCI.20-05-01791.2000","article-title":"NMDA receptor\u2010mediated subthreshold Ca2+ signals in spines of hippocampal neurons","volume":"20","author":"Kovalchuk Y","year":"2000","journal-title":"J Neurosci"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02888.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020708"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.1.193"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.03.001"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/73868"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0953-816X.2004.03380.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"4325","DOI":"10.1523\/JNEUROSCI.19-11-04325.1999","article-title":"Calcium\u2010induced calcium release contributes to action potential\u2010evoked calcium transients in hippocampal CA1 pyramidal neurons","volume":"19","author":"Sandler VM","year":"1999","journal-title":"J Neurosci"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858409355829"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-01-00190.1997"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80698-4"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00004.2004"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5528-08.2009"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2011.217315","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2011.217315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:38:38Z","timestamp":1698777518000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2011.217315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,14]]},"references-count":25,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2011,12,15]]}},"alternative-id":["10.1113\/jphysiol.2011.217315"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2011.217315","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,14]]}}}