{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:17:33Z","timestamp":1790702253933,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T00:00:00Z","timestamp":1333411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p><jats:bold>Key points\u2002<\/jats:bold> <jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The high cation permeability in red blood cells (RBCs) from patients with sickle cell disease (SCD) is central to pathogenesis and includes a deoxygenation\u2010induced pathway termed <jats:italic>P<\/jats:italic><jats:sub>sickle<\/jats:sub>.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Here whole\u2010cell patch clamp configuration was used to record from RBCs of SCD patients and showed a conductance reversibly induced upon deoxygenation, permeable to univalent (Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup>, Rb<jats:sup>+<\/jats:sup>) and divalent (Ca<jats:sup>2+<\/jats:sup>, Mg<jats:sup>2+<\/jats:sup>) cations, and sensitive to tarantula spider toxin GsMTx\u20104, Mn<jats:sup>2+<\/jats:sup> and <jats:italic>o<\/jats:italic>\u2010vanillin.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Divalent cation permeability is particularly important as entry of Ca<jats:sup>2+<\/jats:sup> stimulates the Gardos channel whilst Mg<jats:sup>2+<\/jats:sup> loss will stimulate KCl cotransport.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In oxygenated RBCs, the conductance was pH sensitive, increasing as pH fell from 7.4 to 6, but unaffected when pH was raised from 7.4 to 8.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Results show a conductance that shares many features with the <jats:italic>P<\/jats:italic><jats:sub>sickle<\/jats:sub> flux pathway, and indicating its possible identity as a stretch\u2010activated channel with activation requiring sickle cell haemoglobin (HbS) polymerisation.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1113\/jphysiol.2012.229609","type":"journal-article","created":{"date-parts":[[2012,3,13]],"date-time":"2012-03-13T04:23:53Z","timestamp":1331612633000},"page":"2095-2105","source":"Crossref","is-referenced-by-count":34,"title":["The conductance of red blood cells from sickle cell patients: ion selectivity and inhibitors"],"prefix":"10.1113","volume":"590","author":[{"given":"Y.\u2010L.","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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