{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:39:49Z","timestamp":1790185189741,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2012,7,23]],"date-time":"2012-07-23T00:00:00Z","timestamp":1343001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Key points<\/jats:title>\n                  <jats:p>\n                    <jats:list list-type=\"bullet\">\n                      <jats:list-item>\n                        <jats:p>Carotid body (CB) chemoreceptor complexes consist of receptor type I cells, intimately associated with glia\u2010like type II cells whose function is poorly understood.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>We show that type II cells in the rat CB express gap junction\u2010like proteins, pannexin\u20101 (Panx\u20101) channels, which form non\u2010selective pores permeable to ions and large molecules such as ATP, a key CB neurotransmitter.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>\n                          Activation of purinergic P2Y2 receptors on type II cells led to a rise in intracellular Ca\n                          <jats:sup>2+<\/jats:sup>\n                          , and a prolonged membrane depolarization due to opening of Panx\u20101 channels.\n                        <\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>In a CB co\u2010culture model, where purinergic P2X2\/3\u2010expressing petrosal neurones served as a reporter or biosensor of ATP release, we show that selective activation of P2Y2 receptors on type II cells can lead to ATP release via Panx\u20101 channels.<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>We propose that type II cells may function as amplifiers of the neurotransmitter ATP during chemotransduction, via the mechanism of ATP\u2010induced ATP release.<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Abstract\u2002<\/jats:bold>\n                    Signal processing in the carotid body (CB) is initiated at receptor glomus (or type I) cells which depolarize and release the excitatory neurotransmitter ATP during chemoexcitation by hypoxia and acid hypercapnia. Glomus cell clusters (GCs) occur in intimate association with glia\u2010like type II cells which express purinergic P2Y2 receptors (P2Y2Rs) but their function is unclear. Here we immunolocalize the gap junction\u2010like protein channel pannexin\u20101 (Panx\u20101) in type II cells and show Panx\u20101 mRNA expression in the rat CB. As expected, type II cell activation within or near isolated GCs by P2Y2R agonists, ATP and UTP (100 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    ), induced a rise in intracellular [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]. Moreover in perforated\u2010patch whole cell recordings from type II cells, these agonists caused a prolonged depolarization and a concentration\u2010dependent, delayed opening of non\u2010selective ion channels that was prevented by Panx\u20101 blockers, carbenoxolone (5 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    ) and 4,4\u2032\u2010diisothiocyano\u20102,2\u2032\u2010stilbenedisulfonic acid (DIDS; 10 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    ). Because Panx\u20101 channels serve as conduits for ATP release, we hypothesized that paracrine, type II cell P2Y2R activation leads to ATP\u2010induced ATP release. In proof\u2010of\u2010principle experiments we used co\u2010cultured chemoafferent petrosal neurones (PNs), which express P2X2\/3 purinoceptors, as sensitive biosensors of ATP released from type II cells. In several cases, UTP activation of type II cells within or near GCs led to depolarization or increased firing in nearby PNs, and the effect was reversibly abolished by the selective P2X2\/3 receptor blocker, pyridoxalphosphate\u20106\u2010azophenyl\u20102\u2032,4\u2032\u2010disulphonic acid (PPADS; 10 \u03bc\n                    <jats:sc>m<\/jats:sc>\n                    ). We propose that CB type II cells may function as ATP amplifiers during chemotransduction via paracrine activation of P2Y2Rs and Panx\u20101 channels.\n                  <\/jats:p>","DOI":"10.1113\/jphysiol.2012.236265","type":"journal-article","created":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T14:26:43Z","timestamp":1340720803000},"page":"4335-4350","source":"Crossref","is-referenced-by-count":77,"title":["P2Y2 receptor activation opens pannexin\u20101 channels in rat carotid body type II cells: potential role in amplifying the neurotransmitter ATP"],"prefix":"10.1113","volume":"590","author":[{"given":"Min","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikol A.","family":"Piskuric","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy","family":"Vollmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin 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