{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:57:27Z","timestamp":1791349047553,"version":"4.3.1"},"reference-count":51,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,2,21]],"date-time":"2014-02-21T00:00:00Z","timestamp":1392940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008530","name":"FEDER","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["FCOMP-01-0124-FEDER-029623"],"award-info":[{"award-number":["FCOMP-01-0124-FEDER-029623"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/NEU-SCC\/0347\/2012"],"award-info":[{"award-number":["PTDC\/NEU-SCC\/0347\/2012"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["PTDC\/NEU-NMC\/0494\/2012"],"award-info":[{"award-number":["PTDC\/NEU-NMC\/0494\/2012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Spinal lamina I is a key element of the pain processing system which relays peripheral inputs to supraspinal areas.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In this study, we focused on signal processing in identified lamina I local\u2010circuit and projection neurones in their functionally preserved network in an isolated spinal cord preparation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We found that local\u2010circuit neurones generate spontaneous rhythmic firing, which persists in the presence of blockers of fast synaptic transmission.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We describe a novel, low\u2010threshold, primary afferent\u2010driven inhibitory input to lamina I neurones, which temporally preceded classical high\u2010threshold excitatory inputs and may function as a postsynaptic gate controlling pain.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>One\u2010third of local\u2010circuit neurones and two\u2010thirds of projection neurones responded to substance P application.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These results help us understand the role of lamina I neurones in a new circuit for gating pain responses.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Spinal lamina I is a key element of the pain processing system which relays primary afferent input to supraspinal areas. However, little is known about how the signal is modulated by its intrinsic network including local\u2010circuit neurones (LCNs) and much less numerous anterolateral tract projection neurones (PNs). Here, we used whole\u2010cell patch clamp recordings in an isolated spinal cord preparation to examine properties of identified LCNs (<jats:italic>n<\/jats:italic>\u00a0=\u00a085) and PNs (<jats:italic>n<\/jats:italic>\u00a0=\u00a073) in their functionally preserved local networks. Forty LCNs showed spontaneous rhythmic firing (2\u20137\u00a0Hz) at zero current injection, which persisted in the presence of blockers of fast synaptic transmission. In the remaining cases, most LCNs and PNs fired tonically in response to depolarizing current injections. We identified LCNs and PNs receiving low\u2010threshold primary afferent\u2010driven inhibitory inputs, which in many cases were disynaptic and temporally preceded classical high\u2010threshold excitatory inputs. This direct inhibitory link between low\u2010threshold afferents and PNs can function as a postsynaptic gate controlling the nociceptive information flow in the spinal cord. The LCNs were found to be integrated into the superficial dorsal horn network by their receipt of monosynaptic and disynaptic inputs from other lamina I and II neurones. One\u2010third of LCNs and two\u2010thirds of PNs tested responded to substance P application. Thus, substance P released by a noxious afferent stimulation may excite PNs in two ways: directly, and via the activation of presynaptic LCN circuitries. In conclusion, we have described important properties of identified lamina I neurones and their roles in a new circuit for gating pain responses.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jphysiol.2013.269472","type":"journal-article","created":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T02:54:43Z","timestamp":1389668083000},"page":"1519-1534","source":"Crossref","is-referenced-by-count":35,"title":["Peripherally driven low\u2010threshold inhibitory inputs to lamina I local\u2010circuit and projection neurones: a new circuit for gating pain responses"],"prefix":"10.1113","volume":"592","author":[{"given":"Liliana L.","family":"Luz","sequence":"first","affiliation":[{"name":"Neuronal Networks Group Institute of Molecular and Cellular Biology (IBMC)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Szucs","sequence":"additional","affiliation":[{"name":"Neuronal Networks Group Institute of Molecular and Cellular Biology (IBMC)"},{"name":"Laboratory of Cellular and Molecular Biology Faculty of Medicine University of Porto  Porto Portugal"},{"name":"Department of Physiology University of Debrecen  Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris V.","family":"Safronov","sequence":"additional","affiliation":[{"name":"Neuronal Networks Group Institute of Molecular and Cellular Biology 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