{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:43:44Z","timestamp":1773290624303,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T00:00:00Z","timestamp":1402358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Natural Sciences and Engineering Research Council of Canada (NSERC)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2014,7,15]]},"abstract":"<jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Hydrogen sulphide (H<jats:sub>2<\/jats:sub>S), a gaseous neurotransmitter, is involved in oxygen sensing in glomus cells, which are oxygen\u2010sensing cells found in the mammalian carotid body.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neuroepithelial cells (NECs) are oxygen\u2010sensing cells of fish and are thought to be phylogenetic precursors of mammalian glomus cells; however, the oxygen\u2010sensing mechanisms of these cells remain largely unknown.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Both adult and larval zebrafish responded to exogenous H<jats:sub>2<\/jats:sub>S by increasing ventilation in a dose\u2010dependent manner; H<jats:sub>2<\/jats:sub>S increased intracellular [Ca<jats:sup>2+<\/jats:sup>] in NECs.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Inhibiting endogenous H<jats:sub>2<\/jats:sub>S production decreased or abolished the ventilatory response to hypoxia in both adult and larval zebrafish.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results demonstrate an important role for H<jats:sub>2<\/jats:sub>S in oxygen sensing in zebrafish.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>The current study investigated the role of hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) in oxygen sensing, intracellular signalling and promotion of ventilatory responses to hypoxia in adult and larval zebrafish (<jats:italic>Danio rerio<\/jats:italic>). Both larval and adult zebrafish exhibited a dose\u2010dependent increase in ventilation to sodium sulphide (Na<jats:sub>2<\/jats:sub>S), an H<jats:sub>2<\/jats:sub>S donor. In vertebrates, cystathionine \u03b2\u2010synthase (CBS) and cystathionine \u03b3\u2010lyase (CSE) are enzymes that catalyse the endogenous production of H<jats:sub>2<\/jats:sub>S. In adult zebrafish, inhibition of both CBS and CSE with aminooxyacetate (AOA) and propargyl glycine (PPG) blunted or abolished the hypoxic hyperventilation, and the addition of Na<jats:sub>2<\/jats:sub>S to the water partially rescued the effects of inhibiting endogenous H<jats:sub>2<\/jats:sub>S production. In zebrafish larvae (4\u00a0days post\u2010fertilization), gene knockdown of either CBS or CSE using morpholinos attenuated the hypoxic ventilatory response. Furthermore, the intracellular calcium concentration of isolated neuroepithelial cells (NECs), which are putative oxygen chemoreceptors, increased significantly when these cells were exposed to 50\u00a0\u03bc<jats:sc>m<\/jats:sc> Na<jats:sub>2<\/jats:sub>S, supporting a role for H<jats:sub>2<\/jats:sub>S in Ca<jats:sup>2+<\/jats:sup>\u2010evoked neurotransmitter release in these cells. Finally, immunohistochemical labelling showed that NECs dissociated from adult gill contained CBS and CSE, whereas cutaneous NECs in larval zebrafish expressed only CSE. Taken together, these data show that H<jats:sub>2<\/jats:sub>S can be produced in the putative oxygen\u2010sensing cells of zebrafish, the NECs, in which it appears to play a pivotal role in promoting the hypoxic ventilatory response.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jphysiol.2014.271098","type":"journal-article","created":{"date-parts":[[2014,4,23]],"date-time":"2014-04-23T00:28:12Z","timestamp":1398212892000},"page":"3075-3088","source":"Crossref","is-referenced-by-count":54,"title":["The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (<i>Danio rerio<\/i>)"],"prefix":"10.1113","volume":"592","author":[{"given":"Cosima S.","family":"Porteus","sequence":"first","affiliation":[{"name":"Department of Biosciences University of British Columbia  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara J.","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Department of Biology University of Ottawa  Ottawa ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Pollack","sequence":"additional","affiliation":[{"name":"Department of Biology University of Ottawa  Ottawa ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Kumai","sequence":"additional","affiliation":[{"name":"Department of Biology University of Ottawa  Ottawa ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond W. 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