{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:25:03Z","timestamp":1777389903653,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T00:00:00Z","timestamp":1406764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Australian Research Council and the National Health & Medical Research Council"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"Key points<\/jats:title>\nProlonged Ca2+<\/jats:sup> transients and Ca2+<\/jats:sup> waves have been observed in mammalian skeletal muscle upon reducing the normal resting inhibition of the ryanodine receptor (RyR)\/Ca2+<\/jats:sup> release channels of the sarcoplasmic reticulum (SR) by lowering cytoplasmic [Mg2+<\/jats:sup>] ([Mg2+<\/jats:sup>]cyto<\/jats:sub>). The mechanism driving Ca2+<\/jats:sup> release under these conditions may have pathophysiological implications but is poorly understood.<\/jats:p><\/jats:list-item>\nThe lowering of [Mg2+<\/jats:sup>]cyto<\/jats:sub> induced a leak of Ca2+<\/jats:sup> from the SR that triggered a local reduction of the SR Ca2+<\/jats:sup> buffering power to induce the rise in [Ca2+<\/jats:sup>]SR<\/jats:sub>, an intra\u2010SR Ca2+<\/jats:sup> transient. Prolonged Ca2+<\/jats:sup> transients always developed following this event and evolved as propagating Ca2+<\/jats:sup> waves. No classical hallmarks of cytoplasmic Ca2+<\/jats:sup> propagation mechanisms were observed in conjunction with these waves.<\/jats:p><\/jats:list-item>\nIn conjunction with the spread of Ca2+<\/jats:sup> release, Ca2+<\/jats:sup> was observed to diffuse through the SR away from the site of the intra\u2010SR Ca2+<\/jats:sup> transient. Ca2+<\/jats:sup> release was sustained by the action of SR Ca2+<\/jats:sup> pumps, presumably to maintain [Ca2+<\/jats:sup>]SR<\/jats:sub> above an inactivation threshold for closure of the RyRs. Inactivation of prolonged Ca2+<\/jats:sup> release allowed [Ca2+<\/jats:sup>]SR<\/jats:sub> to recover to threshold for activation of further Ca2+<\/jats:sup> waves, which were always briefer in duration than the initial Ca2+<\/jats:sup> release transients, consistent with a reduced SR Ca2+<\/jats:sup> buffering capacity.<\/jats:p><\/jats:list-item>\nThese observations reveal activation of Ca2+<\/jats:sup> release in mammalian skeletal muscle by [Ca2+<\/jats:sup>]SR<\/jats:sub> in the absence of the normal resting inhibition of the RyR and changes in voltage. Diffusion of Ca2+<\/jats:sup> within SR promotes propagation of Ca2+<\/jats:sup> release.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>Abstract<\/jats:title>Skeletal muscle fibres are large and highly elongated cells specialized for producing the force required for posture and movement. The process of controlling the production of force within the muscle, known as excitation\u2013contraction coupling, requires virtually simultaneous release of large amounts of Ca2+<\/jats:sup> from the sarcoplasmic reticulum (SR) at the level of every sarcomere within the muscle fibre. Here we imaged Ca2+<\/jats:sup> movements within the SR, tubular (t\u2010) system and in the cytoplasm to observe that the SR of skeletal muscle is a connected network capable of allowing diffusion of Ca2+<\/jats:sup> within its lumen to promote the propagation of Ca2+<\/jats:sup> release throughout the fibre under conditions where inhibition of SR ryanodine receptors (RyRs) was reduced. Reduction of cytoplasmic [Mg2+<\/jats:sup>] ([Mg2+<\/jats:sup>]cyto<\/jats:sub>) induced a leak of Ca2+<\/jats:sup> through RyRs, causing a reduction in SR Ca2+<\/jats:sup> buffering power argued to be due to a breakdown of SR calsequestrin polymers, leading to a local elevation of [Ca2+<\/jats:sup>]SR<\/jats:sub>. The local rise in [Ca2+<\/jats:sup>]SR<\/jats:sub>, an intra\u2010SR Ca2+<\/jats:sup> transient, induced a local diffusely rising [Ca2+<\/jats:sup>]cyto<\/jats:sub>. A prolonged Ca2+<\/jats:sup> wave lasting tens of seconds or more was generated from these events. Ca2+<\/jats:sup> waves were dependent on the diffusion of Ca2+<\/jats:sup> within the lumen of the SR and ended as [Ca2+<\/jats:sup>]SR<\/jats:sub> dropped to low levels to inactivate RyRs. Inactivation of RyRs allowed re\u2010accumulation of [Ca2+<\/jats:sup>]SR<\/jats:sub> and the activation of secondary Ca2+<\/jats:sup> waves in the persistent presence of low [Mg2+<\/jats:sup>]cyto<\/jats:sub> if the threshold [Ca2+<\/jats:sup>]SR<\/jats:sub> for RyR opening could be reached. Secondary Ca2+<\/jats:sup> waves occurred without an abrupt reduction in SR Ca2+<\/jats:sup> buffering power. Ca2+<\/jats:sup> release and wave propagation occurred in the absence of Ca2+<\/jats:sup>\u2010induced Ca2+<\/jats:sup> release. These observations are consistent with the activation of Ca2+<\/jats:sup> release through RyRs of lowered cytoplasmic inhibition by [Ca2+<\/jats:sup>]SR<\/jats:sub> or store overload\u2010induced Ca2+<\/jats:sup> release. Restitution of SR Ca2+<\/jats:sup> buffering power to its initially high value required imposing normal resting ionic conditions in the cytoplasm, which re\u2010imposed the normal resting inhibition on the RyRs, allowing [Ca2+<\/jats:sup>]SR<\/jats:sub> to return to endogenous levels without activation of store overload\u2010induced Ca2+<\/jats:sup> release. These results are discussed in the context of how pathophysiological Ca2+<\/jats:sup> release such as that occurring in malignant hyperthermia can be generated.<\/jats:p><\/jats:sec>","DOI":"10.1113\/jphysiol.2014.274274","type":"journal-article","created":{"date-parts":[[2014,6,28]],"date-time":"2014-06-28T04:23:04Z","timestamp":1403929384000},"page":"3727-3746","source":"Crossref","is-referenced-by-count":22,"title":["Activation and propagation of Ca2+<\/sup> release from inside the sarcoplasmic reticulum network of mammalian skeletal muscle"],"prefix":"10.1113","volume":"592","author":[{"given":"Tanya R.","family":"Cully","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences The University of Queensland Brisbane QLD 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua N.","family":"Edwards","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Queensland Brisbane QLD 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley S.","family":"Launikonis","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences The University of Queensland Brisbane QLD 4072 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(72)90194-6"},{"key":"e_1_2_6_3_1","first-page":"w13652","article-title":"Malignant hyperthermia","volume":"142","author":"Bandschapp O","year":"2012","journal-title":"Swiss Med 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