{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:25:04Z","timestamp":1792787104658,"version":"4.5.0"},"reference-count":75,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T00:00:00Z","timestamp":1414627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Health","award":["HL096552"],"award-info":[{"award-number":["HL096552"]}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2014,12,15]]},"abstract":"<jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Lymph transport is promoted by lymphatic pumping, a robust phasic contractile activity of the collecting lymphatic vessels. This contractile function, critical for tissue fluid homeostasis and immune cell transport to lymph nodes, is regulated by the amount of lymph entering the vessels and subsequent distension of the vessel wall.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            While lymphatic pumping relies on influx of Ca\n                            <jats:sup>2+<\/jats:sup>\n                            through voltage\u2010dependent Ca\n                            <jats:sup>2+<\/jats:sup>\n                            channels, characterization of these channels and details of their contribution to the regulation of stretch\u2010activated contractions are lacking.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Here we report the expression of L\u2010 and T\u2010type Ca\n                            <jats:sup>2+<\/jats:sup>\n                            channels in rat mesenteric lymphatic vessels and their differential role in regulating strength and frequency of lymphatic contractions.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>This study fosters our knowledge on the mechanisms that drive stretch\u2010activated lymphatic contractions. It may help in providing a basis to developing agents able to enhance lymphatic function, which could be of therapeutic benefit during lymphatic impairment such as lymphoedema.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Lymph drainage maintains tissue fluid homeostasis and facilitates immune response. It is promoted by phasic contractions of collecting lymphatic vessels through which lymph is propelled back into the blood circulation. This rhythmic contractile activity (i.e. lymphatic pumping) increases in rate with increase in luminal pressure and relies on activation of nifedipine\u2010sensitive voltage\u2010dependent Ca\n                      <jats:sup>2+<\/jats:sup>\n                      channels (VDCCs). Despite their importance, these channels have not been characterized in lymphatic vessels. We used pressure\u2010 and wire\u2010myography as well as intracellular microelectrode electrophysiology to characterize the pharmacological and electrophysiological properties of L\u2010type and T\u2010type VDCCs in rat mesenteric lymphatic vessels and evaluated their particular role in the regulation of lymphatic pumping by stretch. We complemented our study with PCR and confocal immunofluorescence imaging to investigate the expression and localization of these channels in lymphatic vessels. Our data suggest a delineating role of VDCCs in stretch\u2010induced lymphatic vessel contractions, as the stretch\u2010induced increase in force of lymphatic vessel contractions was significantly attenuated in the presence of L\u2010type VDCC blockers nifedipine and diltiazem, while the stretch\u2010induced increase in contraction frequency was significantly decreased by the T\u2010type VDCC blockers mibefradil and nickel. The latter effect was correlated with a hyperpolarization. We propose that activation of T\u2010type VDCCs depolarizes membrane potential, regulating the frequency of lymphatic contractions via opening of L\u2010type VDCCs, which drive the strength of contractions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jphysiol.2014.280347","type":"journal-article","created":{"date-parts":[[2014,10,18]],"date-time":"2014-10-18T01:52:55Z","timestamp":1413597175000},"page":"5409-5427","source":"Crossref","is-referenced-by-count":71,"title":["Distinct roles of L\u2010 and T\u2010type voltage\u2010dependent Ca\n                    <sup>2+<\/sup>\n                    channels in regulation of lymphatic vessel contractile activity"],"prefix":"10.1113","volume":"592","author":[{"given":"Stewart","family":"Lee","sequence":"first","affiliation":[{"name":"Inflammation Research Network and Smooth Muscle Research Group Snyder Institute for Chronic Diseases Department of Physiology &amp; Pharmacology Cumming School of Medicine University of Calgary Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Roizes","sequence":"additional","affiliation":[{"name":"Inflammation Research Network and Smooth Muscle Research Group Snyder Institute for Chronic Diseases Department of Physiology &amp; Pharmacology Cumming School of Medicine University of Calgary Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre\u2010Yves","family":"von der Weid","sequence":"additional","affiliation":[{"name":"Inflammation Research Network and Smooth Muscle Research Group Snyder Institute for Chronic Diseases Department of Physiology &amp; Pharmacology Cumming School of Medicine University of Calgary Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,10,30]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"H479","article-title":"Effect of norepinephrine on 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