{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:32:10Z","timestamp":1759336330843},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1966,6,1]],"date-time":"1966-06-01T00:00:00Z","timestamp":-113184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1966,6]]},"abstract":"<jats:p>1. In cats anaesthetized with intraperitoneal pentobarbitone sodium, three regions of the cerebral ventricles, the third ventricle, the inferior or the anterior horn, were perfused with artificial c.s.f. and the effluent was tested on the fundus strip of the rat's stomach.<\/jats:p><jats:p>2. Effluent from all three regions contracted the fundus strip. The contractions were due to at least two substances as revealed by treatment of the strip with 2\u2010bromolysergic acid diethylamide (BOL). The contractions that were sensitive to BOL are attributed to 5\u2010hydroxytryptamine (5\u2010HT) whereas the BOL resistant contractions appear to be due to an unknown hydroxy acid related to irin or the prostaglandins.<\/jats:p><jats:p>3. The contractions produced by effluent collected from the third ventricle were due wholly or mainly to 5\u2010HT, those from the inferior horn to the unknown hydroxy acid, and those from the anterior horn to both substances in varying proportions. In addition, some samples of effluent from the third ventricle seemed to contain catecholamines as well.<\/jats:p><jats:p>4. The 5\u2010HT in the effluent from the third ventricle is thought to be derived from the hypothalamus. The amounts assayed in 1 ml. effluent\u2014the volume collected during 10 or 20 min perfusion\u2014varied between 0\u00b74 and 12 ng 5\u2010HT. Output of 5\u2010HT was initially high, then usually decreased but sometimes increased again during prolonged perfusion when temperature began to rise as anaesthesia lightened or when additional pentobarbitone sodium was given intravenously.<\/jats:p><jats:p>5. When perfusion of the third ventricle was continued after death the 5\u2010HT content in the effluent increased 3 to 24\u2010fold during the first hour and then gradually declined. This post mortem rise in 5\u2010HT output suggests an abnormal state of release of 5\u2010HT from the hypothalamus. The theory is discussed that the same may happen in certain cases of brain injury and that the abnormal release of 5\u2010HT would explain the pyrexia and shivering seen in such cases.<\/jats:p><jats:p>6. The intraperitoneal injection of 5\u2010hydroxytryptophan greatly increased the output of 5\u2010HT in the effluent from the perfused third ventricle but only when this precursor of 5\u2010HT was injected in large doses which caused respiratory arrest thus necessitating artificial ventilation. Upon the injection of 150 mg\/kg the output of 5\u2010HT rose to 90 ng\/ml. and a further rise to 180 ng\/ml. occurred when perfusion was continued after death.<\/jats:p><jats:p>7. It was not possible to establish a relation between the presence of the hydroxy acid in the effluent from the inferior horn and neuronal activity.<\/jats:p><jats:p>8. The 5\u2010HT detected in the effluent from the anterior horn is assumed to have been released from the caudate nucleus.<\/jats:p>","DOI":"10.1113\/jphysiol.1966.sp007951","type":"journal-article","created":{"date-parts":[[2014,12,20]],"date-time":"2014-12-20T01:48:17Z","timestamp":1419040097000},"page":"837-855","source":"Crossref","is-referenced-by-count":104,"title":["Appearance of 5\u2010hydroxytryptamine and an unidentified pharmacologically active lipid acid in effluent from perfused cerebral ventricles"],"prefix":"10.1113","volume":"184","author":[{"given":"W.","family":"Feldberg","sequence":"first","affiliation":[]},{"given":"R. D.","family":"Myers","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1966,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1966.sp007951","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1966.sp007951","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T16:13:39Z","timestamp":1699460019000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1966.sp007951"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1966,6]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1966,6]]}},"alternative-id":["10.1113\/jphysiol.1966.sp007951"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1966.sp007951","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1966,6]]}}}