{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T14:52:55Z","timestamp":1758120775867},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1971,1,1]],"date-time":"1971-01-01T00:00:00Z","timestamp":31536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1971,1]]},"abstract":"<jats:p>1. The effects of ouabain and diphenylhydantoin (DPH) to inhibit and stimulate, respectively, the Na<jats:sup>+<\/jats:sup>\u2014K<jats:sup>+<\/jats:sup> pump were used to correlate transmembrane resting potentials (RP), ionic gradients, and cell pH (DMO method) in rat muscle and liver <jats:italic>in vivo<\/jats:italic>.<\/jats:p><jats:p>2. Ouabain effects included a rise in K<jats:sup>+<\/jats:sup> and fall in Na<jats:sup>+<\/jats:sup> concentration in plasma, a rise in intracellular Na<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup> and a fall in K<jats:sup>+<\/jats:sup> concentration and pH<jats:sub>i<\/jats:sub> in muscle, and a rise in intracellular K<jats:sup>+<\/jats:sup> concentration in liver.<\/jats:p><jats:p>3. Measured muscle RP was decreased from \u221290 to \u221265 mV by ouabain with the RP predictable from the Goldman equation for Na<jats:sup>+<\/jats:sup> and K<jats:sup>+<\/jats:sup> with <jats:italic>P<\/jats:italic><jats:sub>Na<\/jats:sub>\/<jats:italic>P<\/jats:italic><jats:sub>K<\/jats:sub> = 0\u00b701.<\/jats:p><jats:p>4. Measured hepatic RP was increased from \u221244 to \u221248 mV by ouabain, whereas the Goldman equation predicts the potential should decrease. A change in permeability of some ion or activation of an electrogenic pump component is necessary to explain this result.<\/jats:p><jats:p>5. DPH produced no significant effect on muscle electrolytes or RP and failed to reverse the effect of ouabain at the time measured and in the doses used.<\/jats:p><jats:p>6. DPH produced a slight rise in hepatic cell K<jats:sup>+<\/jats:sup> and a rise from \u221242 to \u221247 mV in hepatic RP. This hyperpolarization also cannot be explained without invoking a permeability change or activation of an electrogenic pump.<\/jats:p><jats:p>In all cases intracellular Cl<jats:sup>\u2212<\/jats:sup> in both muscle and liver changed in the direction expected from the change in the RP. Muscle Cl<jats:sup>\u2212<\/jats:sup> appears passively distributed if a constant amount of extra or bound Cl<jats:sup>\u2212<\/jats:sup> is first subtracted from each group. Hepatic intracellular Cl<jats:sup>\u2212<\/jats:sup> is always less than expected on the basis of passive distribution, although errors in determination do not allow elimination of the possibility that Cl<jats:sup>\u2212<\/jats:sup> distribution is determined only by the RP.<\/jats:p><jats:p>8. Cell pH and RP data were used to calculate H<jats:sup>+<\/jats:sup> gradients. DPH had no effect on cell pH and only slightly increased the H<jats:sup>+<\/jats:sup> gradient in liver. Ouabain produced a slight fall in muscle cell pH but reduced the H<jats:sup>+<\/jats:sup> gradient by half. In liver only the H<jats:sup>+<\/jats:sup> gradient was increased slightly. The data support the concept of a loose coupling between active H<jats:sup>+<\/jats:sup> and Na<jats:sup>+<\/jats:sup>\u2014K<jats:sup>+<\/jats:sup> transport.<\/jats:p>","DOI":"10.1113\/jphysiol.1971.sp009312","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T11:04:55Z","timestamp":1418987095000},"page":"101-115","source":"Crossref","is-referenced-by-count":61,"title":["Effects of ouabain and diphenylhydantoin on transmembrane potentials, intracellular electrolytes and cell pH of rat muscle and liver <i>in vivo<\/i>"],"prefix":"10.1113","volume":"212","author":[{"given":"J. A.","family":"Williams","sequence":"first","affiliation":[]},{"given":"C. D.","family":"Withrow","sequence":"additional","affiliation":[]},{"given":"D. M.","family":"Woodbury","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1971,1]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1971.sp009312","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1971.sp009312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T15:00:36Z","timestamp":1699455636000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1971.sp009312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,1]]}},"alternative-id":["10.1113\/jphysiol.1971.sp009312"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1971.sp009312","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,1]]}}}