{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T11:20:33Z","timestamp":1774437633653,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1971,5,1]],"date-time":"1971-05-01T00:00:00Z","timestamp":41904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1971,5]]},"abstract":"<jats:p>1. In cats under methoxyflurane, DNP and other metabolic inhibitors were tested on cortical neurones by iontophoresis from micropipettes.<\/jats:p><jats:p>2. DNP, dinitro\u2010<jats:italic>o<\/jats:italic>\u2010cresol, iodoacetate, pentachlorophenol and oligomycin (uncouplers or inhibitors of oxidative phosphorylation), as well as moderate anoxia, blocked selectively and reversibly spontaneous firing and discharges evoked by ACh; responses evoked by glutamate were facilitated by moderate doses of DNP and blocked only by large amounts.<\/jats:p><jats:p>3. Azide, cyanide, ouabain and strophanthidine had a mainly excitatory effect; the cardiac glycosides tended to depress more strongly responses to glutamate.<\/jats:p><jats:p>4. Intracellular observations showed that DNP causes a sharp fall in electrical excitability, associated with a hyperpolarization and fall in membrane resistance.<\/jats:p><jats:p>5. The hyperpolarizing action of DNP had a mean reversal level (<jats:italic>E<\/jats:italic><jats:sub>DNP<\/jats:sub>) nearly 30 mV more negative than the resting potential; <jats:italic>E<\/jats:italic><jats:sub>DNP<\/jats:sub> was identical with the mean reversal level for the depolarizing action of ACh, measured on the same cells.<\/jats:p><jats:p>6. DNP had its usual hyperpolarizing effect on neurones whose IPSPs had been made positive by raising the internal [Cl]; the mean <jats:italic>E<\/jats:italic><jats:sub>IPSP<\/jats:sub> was over 30 mV more positive than <jats:italic>E<\/jats:italic><jats:sub>DNP<\/jats:sub>.<\/jats:p><jats:p>7. It is concluded that DNP lowers excitability by raising the membrane conductance to K<jats:sup>+<\/jats:sup> (<jats:italic>g<\/jats:italic><jats:sub>K<\/jats:sub>) and that it blocks ACh responses selectively because ACh has a precisely opposite action on these neurones.<\/jats:p><jats:p>8. In the Discussion, it is suggested that the rise in <jats:italic>g<\/jats:italic><jats:sub>K<\/jats:sub> is mediated by an increase in internal free Ca<jats:sup>2+<\/jats:sup>, caused by a slowing of mitochondrial activity, and that a similar mechanism may play a significant role in general anaesthesia.<\/jats:p>","DOI":"10.1113\/jphysiol.1971.sp009465","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T11:12:02Z","timestamp":1418987522000},"page":"199-222","source":"Crossref","is-referenced-by-count":109,"title":["Actions of dinitrophenol and some other metabolic inhibitors on cortical neurones"],"prefix":"10.1113","volume":"215","author":[{"given":"J. M.","family":"Godfraind","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kawamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Krnjevi\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Pumain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1971,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1971.sp009465","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1971.sp009465","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T18:25:33Z","timestamp":1699467933000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1971.sp009465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,5]]}},"alternative-id":["10.1113\/jphysiol.1971.sp009465"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1971.sp009465","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,5]]}}}