{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T09:53:01Z","timestamp":1715248381953},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1971,10,1]],"date-time":"1971-10-01T00:00:00Z","timestamp":55123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1971,10]]},"abstract":"<jats:p>1. A new technique is presented for determining the volume of extracellular space in bowfin (<jats:italic>Amia calva<\/jats:italic>) brain during <jats:italic>in vitro<\/jats:italic> incubation. It consists of solving simultaneous equations which are applied to determine the volume of extracellular space as well as intracellular marker concentration. This technique allows for a better insight into the redistribution of marker between incubation medium and extracellular space as well as between extracellular and intracellular space.<\/jats:p><jats:p>2. Na<jats:sup>+<\/jats:sup>, K<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup> equilibrated within 10\u201315 min between incubation medium and extracellular space. There was no evidence of a homoeostatic mechanism controlling the concentration of these ions in the extracellular fluid, which appeared to be in equilibrium with cerebrospinal fluid. The extracellular spaces of these ions were identical: Na<jats:sup>+<\/jats:sup>, 23\u00b74; K<jats:sup>+<\/jats:sup>, 23\u00b73 and Cl<jats:sup>\u2212<\/jats:sup>, 23\u00b72%.<\/jats:p><jats:p>3. Sorbitol equilibrated with the extracellular fluid within 45 min and indicated an extracellular space of 22\u00b76%, nearly identical with that for electrolytes.<\/jats:p><jats:p>4. Vastly different \u2018spaces\u2019 were obtained for [<jats:sup>3<\/jats:sup>H]methoxy inulin, which equilibrated within 45 min with a 13% space and [<jats:sup>14<\/jats:sup>C]carboxyl inulin, which showed a 46% space value for only 30 min. The difference may be explained by marker decomposition. The 9% difference between the [<jats:sup>3<\/jats:sup>H]methoxy inulin and sorbitol spaces may be explained by a \u2018packing\u2019 factor attributable to molecular size.<\/jats:p>","DOI":"10.1113\/jphysiol.1971.sp009629","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T11:14:48Z","timestamp":1418987688000},"page":"477-493","source":"Crossref","is-referenced-by-count":9,"title":["A new approach for determining the volume of cerebral extracellular fluid and demonstration of its communication with c.s.f"],"prefix":"10.1113","volume":"218","author":[{"given":"Reinhard L.","family":"Friede","sequence":"first","affiliation":[]},{"given":"Kuo Hao","family":"Hu","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1971,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1971.sp009629","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1971.sp009629","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T13:46:16Z","timestamp":1699451176000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1971.sp009629"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,10]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1971,10]]}},"alternative-id":["10.1113\/jphysiol.1971.sp009629"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1971.sp009629","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,10]]}}}