{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T21:28:56Z","timestamp":1771622936714,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1972,4,1]],"date-time":"1972-04-01T00:00:00Z","timestamp":70934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1972,4]]},"abstract":"<jats:p>The volume responses of the T\u2010system of frog skeletal muscle to isotonic solutions of altered ionic composition, as studied by electron microscopic examination of osmium\u2010fixed tissue, have been re\u2010investigated using aldehyde fixatives.<\/jats:p><jats:p>1. In confirmation of the earlier work we find that the T\u2010tubes swell in frog sartorius muscles which have been soaked in Ringer solution in which the sodium chloride concentration has been reduced to 40 m\n<jats:sc>M<\/jats:sc> and tonicity maintained with sucrose before fixation in osmium.<\/jats:p><jats:p>2. The swelling does not occur in muscles similarly treated, but fixed with acrolein or glutaraldehyde.<\/jats:p><jats:p>3. Swelling of the T\u2010tubes, which has been reported for osmium\u2010fixed muscle following exposure to Ringer in which chloride is replaced by acetate, or reduced and sulphate substituted, does not occur when fixation is by acrolein.<\/jats:p><jats:p>4. Analysis of the data from the earlier work with sucrose substitution shows that the degree of T\u2010tube swelling is proportional to the sucrose concentration of the soaking medium, whether the solution be isotonic or anisotonic.<\/jats:p><jats:p>5. It is concluded that the increase in T\u2010tube volume arises in muscles which have been exposed to certain impermeant solutes <jats:italic>during<\/jats:italic> fixation by osmium, and a possible mechanism to account for the effect is described.<\/jats:p><jats:p>6. The relevance of these observations to the hypothesis that the sarcoplasmic reticulum is an extracellular compartment in muscle and to the proposal that the T\u2010tubes may represent an intermediate compartment for potassium fluxes is discussed.<\/jats:p>","DOI":"10.1113\/jphysiol.1972.sp009789","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:36:11Z","timestamp":1418985371000},"page":"95-111","source":"Crossref","is-referenced-by-count":20,"title":["An analysis of volume changes in the T\u2010tubes of frog skeletal muscle exposed to sucrose"],"prefix":"10.1113","volume":"222","author":[{"given":"R. I.","family":"Birks","sequence":"first","affiliation":[]},{"given":"D. F.","family":"Davey","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1972,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1972.sp009789","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1972.sp009789","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T09:48:35Z","timestamp":1699436915000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1972.sp009789"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1972,4]]}},"alternative-id":["10.1113\/jphysiol.1972.sp009789"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1972.sp009789","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,4]]}}}