{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T21:26:37Z","timestamp":1771622797699,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1972,7,1]],"date-time":"1972-07-01T00:00:00Z","timestamp":78796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1972,7]]},"abstract":"<jats:p>1. The rate of inhibition of the Na pump by ouabain was examined both by direct measurement of the rate of decline of the Na efflux and by the binding of [<jats:sup>3<\/jats:sup>H]ouabain.<\/jats:p><jats:p>2. The onset of inhibition of the Na efflux was concentration\u2010dependent; but did not follow simple first order kinetics. The time course of inhibition was roughly exponential although in about 30% of the axons inhibition was preceded by a transient stimulation of the Na efflux.<\/jats:p><jats:p>3. Inhibition of the Na efflux by both ouabain and strophanthidin was apparently irreversible.<\/jats:p><jats:p>4. The onset of inhibition was slowed markedly at low temperatures.<\/jats:p><jats:p>5. Replacement of external Na by choline, dextrose or potassium slowed the rate of inhibition. Li behaved like Na and inhibition was faster in K\u2010ASW than in choline\u2010ASW.<\/jats:p><jats:p>6. The rate of inhibition of Na\u2010Na exchange was similar to that of Na\u2010K exchange, but ouabain failed to bind securely to fully poisoned axons.<\/jats:p><jats:p>7. Two components of [<jats:sup>3<\/jats:sup>H]ouabain\u2010binding could be distinguished. A linear component which probably reflects uptake into the cells and a saturable component which seems to reflect binding to Na\u2010pumping sites.<\/jats:p><jats:p>8. The saturable component of binding followed a similar time course to the inhibition of the Na efflux and the rate of binding was reduced in choline\u2010ASW and in fully poisoned axons.<\/jats:p><jats:p>9. Measurements of [<jats:sup>3<\/jats:sup>H]ouabain\u2010binding indicate that the number of Na pumping sites in the axon membrane is probably between 10<jats:sup>3<\/jats:sup> and 10<jats:sup>4<\/jats:sup>\/\u03bc<jats:sup>2<\/jats:sup>.<\/jats:p>","DOI":"10.1113\/jphysiol.1972.sp009905","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:45:55Z","timestamp":1418985955000},"page":"463-475","source":"Crossref","is-referenced-by-count":42,"title":["Inhibition of the sodium pump in squid axons by cardiac glycosides: dependence on extracellular ions and metabolism"],"prefix":"10.1113","volume":"224","author":[{"given":"P. F.","family":"Baker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. S.","family":"Willis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1972,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1972.sp009905","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1972.sp009905","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T19:01:29Z","timestamp":1699470089000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1972.sp009905"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,7]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1972,7]]}},"alternative-id":["10.1113\/jphysiol.1972.sp009905"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1972.sp009905","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,7]]}}}