{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:57:15Z","timestamp":1770969435032,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1973,11,1]],"date-time":"1973-11-01T00:00:00Z","timestamp":120960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1973,11]]},"abstract":"<jats:p>1. The effects of acetylcholine upon the output of amylase, Ca<jats:sup>2+<\/jats:sup> efflux and membrane potential of pancreatic acinar cells have been measured in segments of mouse pancreas superfused <jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p>2. Amylase output was measured continuously using an on\u2010line automated fluorimetric method; Ca<jats:sup>2+<\/jats:sup> efflux was monitored by measuring the release of <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> from pre\u2010labelled tissue; and intracellular recordings of acinar transmembrane potentials were obtained with glass micro\u2010electrodes. In some experiments membrane potentials, and in others <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux, were measured concomitantly with amylase release.<\/jats:p><jats:p>3. Acetylcholine depolarized the acinar cells, increased tissue <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux and raised amylase output, each with a similar dose\u2010dependence, i.e. a maximal response at 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>, threshold \u22dc 10<jats:sup>\u22128<\/jats:sup> \n<jats:sc>M<\/jats:sc>, and ED<jats:sub>50<\/jats:sub> values of 0\u00b77 \u00d7 10<jats:sup>\u22127<\/jats:sup> \n<jats:sc>M<\/jats:sc>, 0\u00b75 \u00d7 10<jats:sup>\u22127<\/jats:sup> \n<jats:sc>M<\/jats:sc>, and 2 \u00d7 10<jats:sup>\u22127<\/jats:sup> \n<jats:sc>M<\/jats:sc> for depolarization, amylase release, and <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux, respectively.<\/jats:p><jats:p>4. In response to acetylcholine both depolarization and <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux preceded or coincided with the increase in amylase output.<\/jats:p><jats:p>5. Acetylcholine 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc> and [K]<jats:sub>0<\/jats:sub> 47 m\n<jats:sc>M<\/jats:sc> were without effect on <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> efflux in the presence of atropine (3 \u00d7 10<jats:sup>\u22126<\/jats:sup> \n<jats:sc>M<\/jats:sc>) but pancreozymin (0\u00b73 u.\/ml.) still elicited a marked increase in <jats:sup>45<\/jats:sup>Ca<jats:sup>2+<\/jats:sup> release.<\/jats:p><jats:p>6. These results suggest that the stimulatory action of acetylcholine on the pancreatic acinar cell involves, sequentially, a specific receptor\u2010activated increase in membrane permeability, depolarization, Ca<jats:sup>2+<\/jats:sup> mobilization and amylase release. These events are discussed in relation to the integrated mechanism of stimulus\u2010secretion coupling.<\/jats:p>","DOI":"10.1113\/jphysiol.1973.sp010367","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:26:42Z","timestamp":1418984802000},"page":"689-701","source":"Crossref","is-referenced-by-count":120,"title":["Pancreatic acinar cells: acetylcholine\u2010induced membrane depolarization, calcium efflux and amylase release"],"prefix":"10.1113","volume":"234","author":[{"given":"E. K.","family":"Matthews","sequence":"first","affiliation":[]},{"given":"O. H.","family":"Petersen","sequence":"additional","affiliation":[]},{"given":"J. A.","family":"Williams","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1973,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1973.sp010367","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1973.sp010367","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T15:34:44Z","timestamp":1699457684000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1973.sp010367"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,11]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1973,11]]}},"alternative-id":["10.1113\/jphysiol.1973.sp010367"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1973.sp010367","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,11]]}}}