{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T06:52:53Z","timestamp":1769237573698,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1976,11,1]],"date-time":"1976-11-01T00:00:00Z","timestamp":215654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1976,11]]},"abstract":"<jats:p>Single myelinated nerve fibres of Rana esculenta were investigated under voltage clamp conditions at 13 degrees C. Fluctuations of steady\u2010state membrane current were measured during the last 152 msec of 190\u2010225 msec pulses depolarizing the membrane by 8\u201048 mV. Noise power spectral densities were calculated in the frequency range of 6\u20106\u20106757 Hz. 2. External application of 150 nM tetrodotoxin (TTX) and\/or 10 mM tetraethylammonium (TEA) ion reduced the current fluctuations. The difference of current noise spectra measured in the presence and absence of TTX (TEA) was not changed by the presence of TEA (TTX) during both measurements, and was taken as the spectrum of the Na (K) current fluctuations. 3. Residual current noise during application of both TTX and TEA was, except for some excess noise at the low and high frequency ends of the spectrum, similar to the noise measured from a passive nerve model and could be understood in terms of Nyquist noise of the known resistances and the amplifier noise. 4. Na current fluctuation spectra were interpreted as the sum N\/f+SNa(f) where SNa(F) represents the spectrum expected for a set of equal, independent Na channels with only two conductance states (open or closed) which follow Hodgkin\u2010Huxley kinetics. With values of hinfinity, tauh and minfinity measured from macroscopic Na currents, the measured spectra were fitted well by optimizing N, SNa(0) and taum. Values of taum obtained by this method were in fair agreement with values found from macroscopic currents. 5. The 1\/f component of Na current noise was roughly proportional to the square of the steady\u2010state Na current, I2. The mean value of N\/I2 was (1\u20101 +\/\u2010 0\u20103) X 10(\u20104). 6. The current carried by a single Na channel was calculated from fitted spectra and steady\u2010state Na currents measured simultaneously with the current fluctuations. The single channel conductance gamma normalized to zero absolute membrane potential was calculated. The average gamma from twelve measurements at depolarizations of 8\u201040 mV was 7\u20109 +\/\u2010 0\u20109 pS (S.E. of mean). The apparent value of gamma was smallest with small depolarizations. Variations of the assumed kinetic properties of the model did not drastically affect the single channel conductance. 7. External application of 0\u20101 mM\u2010Ni ion lengthened taum in the macroscopic currents and in the fluctuation spectra and enhanced both the steady\u2010state Na current and the current fluctuations. In Ni\u2010treated nodes gamma was smaller than in normal nodes.<\/jats:p>","DOI":"10.1113\/jphysiol.1976.sp011616","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T09:08:59Z","timestamp":1418980139000},"page":"699-727","source":"Crossref","is-referenced-by-count":110,"title":["Measurement of the conductance of the sodium channel from current fluctuations at the node of Ranvier."],"prefix":"10.1113","volume":"262","author":[{"given":"F","family":"Conti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Hille","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Neumcke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Nonner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"St\u00e4mpfli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1976,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1976.sp011616","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1976.sp011616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T08:20:41Z","timestamp":1699258841000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1976.sp011616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,11]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1976,11]]}},"alternative-id":["10.1113\/jphysiol.1976.sp011616"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1976.sp011616","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,11]]}}}