{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:11:47Z","timestamp":1758280307412},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1978,8,1]],"date-time":"1978-08-01T00:00:00Z","timestamp":270777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1978,8]]},"abstract":"<jats:p>1. Frog neuromuscular junctions were viewed with Nomarski optics and voltage clamped. Agonist was applied ionophoretically and agonist concentrations were measured using a micro\u2010electrode sensitive to quaternary amines. 2. The dose\u2010response relationship was studied using the agonists carbamylcholine, suberyldicholine and hydroxyphenyl\u2010propyltrimethylammonium. 3. With all of these agonists, it appeared that the ACh receptor could be active when either one or two agonist  binding sites were occupied. The receptor was much more likely to be active when both sites were occupied. Agonist dissociation constants and receptor activation probabilities were estimated by non\u2010linear regression techniques for several possible receptor activation schemes.<\/jats:p>","DOI":"10.1113\/jphysiol.1978.sp012431","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:53:11Z","timestamp":1418975591000},"page":"421-444","source":"Crossref","is-referenced-by-count":89,"title":["An analysis of the dose\u2010response relationship at voltage\u2010clamped frog neuromuscular junctions."],"prefix":"10.1113","volume":"281","author":[{"given":"V E","family":"Dionne","sequence":"first","affiliation":[]},{"given":"J H","family":"Steinbach","sequence":"additional","affiliation":[]},{"given":"C F","family":"Stevens","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1978,8]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1978.sp012431","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1978.sp012431","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T10:31:31Z","timestamp":1699180291000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1978.sp012431"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,8]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,8]]}},"alternative-id":["10.1113\/jphysiol.1978.sp012431"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1978.sp012431","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,8]]}}}