{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,14]],"date-time":"2024-01-14T23:24:37Z","timestamp":1705274677326},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1978,10,1]],"date-time":"1978-10-01T00:00:00Z","timestamp":276048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1978,10]]},"abstract":"<jats:p>1. Local conductance changes produced by various bath\u2010applied agonists at frog end\u2010plate membrane were measured using focal recording of extracellular potential in voltage\u2010clamped muscle fibres. The potential difference between a focal micropipette placed on the nerve terminal and another micro\u2010pipette placed on or near inactive membrane was taken as proportional to the agonist\u2010induced current through a small patch of an end\u2010plate membrane. 2. The current\u2010voltage (I\u2010\u2010V) relation of active membrane was obtained directly by increasing the membrane potential in a ramp fashion. The change in membrane potential was slow enough for post\u2010synaptic gating processes to reach equilibrium during the ramp. 3. During application of sufficiently low concentrations of full agonists (carbachol, (ACh) and partial agonists (choline and decamethonium) the I\u2010\u2010V relation of end\u2010plate membrane showed strong curvature in the range of \u201060 to \u2010130 mV. The slope of I\u2010\u2010V relations increased exponentially with membrane hyperpolarization, an e\u2010fold change in conductance occurring for about 50 mV potential shift. 4. The curvature of the I\u2010\u2010V relation of end\u2010plate\u2010membrane activated by the partial agonists choline and decamethonium became less as the agonist concentration was increased, and with high concentrations (choline 15 mM; decamethonium 250 micrometer) the I\u2010\u2010V relation became almost straight. 5. When end\u2010plate currents produced by high concentrations of partial agonists were matched by application of equi\u2010active concentrations of carbachol, the carbachol\u2010activated membrane still showed as much curvature in its I\u2010\u2010V relation as when low concentrations of carbachol were used. 6. Choline and decamethonium concentrations for which the I\u2010\u2010V relation was straight produced much greater depression of miniature end\u2010plate currents than did carbachol concentrations which produced the same membrane current at the holding potential. 7. I\u2010\u2010V relations for full agonists at high concentrations were obtained after alpha\u2010bungarotoxin pre\u2010treatment. During application of carbachol (400\u2010\u2010500 micrometer) and ACh (30\u2010\u201040 micrometer; after complete inhibition of acetylcholinesterase activity) the I\u2010\u2010V relation of end\u2010plate membrane is much less curved than during application of low concentrations. 8. It is concluded that either the voltage sensitivity of agonist\u2010induced end\u2010plate conductance reflects voltage sensitivity of agonist binding, or the partial agonists used can exert a voltage\u2010dependent 'local anaesthetic' action in addition to their agonist activity.<\/jats:p>","DOI":"10.1113\/jphysiol.1978.sp012523","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:48:27Z","timestamp":1418975307000},"page":"621-644","source":"Crossref","is-referenced-by-count":26,"title":["A comparison of current\u2010voltage relations for full and partial agonists."],"prefix":"10.1113","volume":"283","author":[{"given":"P R","family":"Adams","sequence":"first","affiliation":[]},{"given":"B","family":"Sakmann","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1978,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1978.sp012523","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1978.sp012523","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T18:03:20Z","timestamp":1699207400000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1978.sp012523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,10]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,10]]}},"alternative-id":["10.1113\/jphysiol.1978.sp012523"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1978.sp012523","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,10]]}}}