{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:19:51Z","timestamp":1759936791138},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1979,4,1]],"date-time":"1979-04-01T00:00:00Z","timestamp":291772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1979,4]]},"abstract":"<jats:p>1. Asymmetrical displacement currents ('gating currents') have been recorded in intracellularly perfused squid giant axons by averaging the currents associated with depolarizing and hyperpolarizing voltage pulses. The effect of temperature on 'gating currents' was studied and compared with the effect of temperature on Na currents. 2. Increasing the temperature in seven steps from 0 to 15 degrees C increased the area under the on\u2010 and off\u2010response (Qon, Qoff). The average Q10 values for Qon and Qoff (measured with depolarizing pulses to 0 to 20 mV) were 1.41 and 1.62, respectively. 3. The on\u2010 and the off\u2010response were described mathematically by the sum of two exponentials. The first component of the on\u2010response, Qon 1, represented 80% or more of the total charge movement associated with 2.5 msec pulses; the Q10 of Qon 1 was similar to that of total Qon. The first component of the off\u2010response, Qoff 1, represented 50\u2010\u201070% of total Qoff; its Q10 was smaller than that of total Qoff. 4. The temperature dependence of the rate constants (tauon 1)\u20101 and (tauoff 1)\u20101 was stronger at temperatures below 6\u2010\u20108 degrees C (Q10 = 3.1\u2010\u20106.4) than at higher temperatures (Q10 = 2.0\u2010\u20103.3). In an Arrhenius plot two lines of different slope were required to fit the data. 5. The effect of increasing the temperature on the Q vs. V curve can be described as an increase of Qmax or, alternatively, as a shift of the curve to more negative potentials. 6. Increasing the temperature from 0 to 15 degrees C increased the peak of the Na current (recorded in sea water with a fifth of the normal Na concentration), increased the rate constants taum\u20101 and tauh\u20101 and shifted the m3infinity and hinfinity curves to more positive potentials. 7. The Q10 of the rate constant taum\u20101 varied between 2.04 and 2.61 and was independent of temperature. In an Arrhenius plot the values for taum\u20101 could be fitted by a single line. 8. The results support the view that 'gating current' does not simply reflect changes of the Na activation variable m. The increase of Qon, Qoff with increasing temperature may be attributed to an increase in membrane fluidity. The possibility that those charges which become mobile at higher temperatures may not be related to gating is considered.<\/jats:p>","DOI":"10.1113\/jphysiol.1979.sp012748","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:31:28Z","timestamp":1418974288000},"page":"479-500","source":"Crossref","is-referenced-by-count":72,"title":["The effect of temperature on the asymmetrical charge movement in squid giant axons."],"prefix":"10.1113","volume":"289","author":[{"given":"J E","family":"Kimura","sequence":"first","affiliation":[]},{"given":"H","family":"Meves","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1979,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1979.sp012748","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1979.sp012748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T03:02:28Z","timestamp":1699239748000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1979.sp012748"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,4]]}},"alternative-id":["10.1113\/jphysiol.1979.sp012748"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1979.sp012748","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,4]]}}}