{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T15:06:39Z","timestamp":1699369599039},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1979,5,1]],"date-time":"1979-05-01T00:00:00Z","timestamp":294364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1979,5]]},"abstract":"<jats:p>1. Theophylline (10 mM) and choleragen change the direction of net Cl\u2010 movements across rabbit ileum, in the short\u2010circuit current condition, from absorption to secretion. The specific activity ratio R of Cl\u2010 tracers within the tissue coming from mucosal and serosal solutions respectively is increased, which is consistent with an increase in Cl\u2010 exchange flux across the mucosal border. 2. Net Na+ movement is also changed from net absorption to secretion by theophylline and choleragen; the specific activity ratio R of Na+ tracers is raised by theophylline. Because of the large paracellular component to transepithelial Na+ movements, an increase in Na+ exchange flux across the mucosal border is not detected. 3. 2,4,6\u2010Triaminopyrimidine (20 mM) which has been previously shown to block paracellular Na+ movements, blocks both the theophylline and choleragen\u2010dependent reversal of net Na+ movement by preventing the decrease in m\u2010s Na flux. The theophylline\u2010dependent increase in the ratio R of Na+ is still present, and is consistent with an increase in Na+ exchange flux across the mucosal border\u2010\u2010unmasked by removal of the paracellular flux components. 4. Ouabain (0.1 mM) abolishes net absorption of Na+ and Cl\u2010 in control and net secretion of Na+ and Cl\u2010 in theophylline\u2010treated tissue. Ouabain does not affect the theophylline\u2010dependent increase in Cl\u2010 exchange across the mucosal border. 5. Replacement of Ringer Cl\u2010 with SO24\u2010 or Na+ by choline prevents the effects of theophylline and choleragen on Na+ and Cl\u2010 fluxes respectively. 6. Ethacrynate (0.1 mM) prevents the theophylline\u2010dependent effects on net Na+ movement. Raising ethacrynate to 0.2 mM abolishes the effects of theophylline on Cl\u2010 exchange. An interpretation of these results is that theophylline and choleragen raise the Cl\u2010 permeability of the brush border. This increases NaCl leakage from the hypertonic lateral intercellular space into the mucosal solution thereby causing secretion. The selective action of triaminopyrimidine and ethacrynate (0.1 mM) on Na+ flux indicates that Na+ and Cl\u2010 move via separate transport pathways across the mucosal border.<\/jats:p>","DOI":"10.1113\/jphysiol.1979.sp012774","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:31:09Z","timestamp":1418974269000},"page":"331-350","source":"Crossref","is-referenced-by-count":27,"title":["The effects of theophylline and choleragen on sodium and chloride ion movements within isolated rabbit ileum."],"prefix":"10.1113","volume":"290","author":[{"given":"R J","family":"Naftalin","sequence":"first","affiliation":[]},{"given":"N L","family":"Simmons","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1979,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1979.sp012774","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1979.sp012774","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T12:02:19Z","timestamp":1699185739000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1979.sp012774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,5]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1979,5]]}},"alternative-id":["10.1113\/jphysiol.1979.sp012774"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1979.sp012774","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,5]]}}}