{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T18:38:51Z","timestamp":1773427131947,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1979,10,1]],"date-time":"1979-10-01T00:00:00Z","timestamp":307584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1979,10]]},"abstract":"<jats:p>1. Liquid ion\u2010exchanger Cl\u2010 \u2010sensitive micro\u2010electrodes were used to make continuous measurements of the intracellular Cl activity, aCli, of quiscent sheep cardiac Purkinje fibres in vitro. 2. aCli was higher than that expected from a passive distribution, (which would have been about 5 mM). It was 3\u2010\u20104 times hiable; EC1 was about 35 mV positive to Em. It was over twice as high in the nominal absence of bicarbonate\/CO2 (when the buffer\u2010system was HEPES\/O2) but was not always so stable, and ECl was about 20 mV positive to Em. 3. Experiments designed to assess the maximum possible error likely to occur in the measurement of aCli showed that this could not be large and that the estimates of ECl were accurate to within 8 mV. 4. The ability of Cl to move down both concentration and potential gradients was established by demonstrating a loss of aCli in Cl\u2010free solutions and a gain when Em was depolarized positive to ECl in high\u2010K solutions. In both cases, the changes were complete within about 100\u2010\u2010160 min. 5. The decline of aCli in Cl\u2010free solutions (glucuronate\u2010substituted) was not significantly affected by changes of [Ca]o from 0 to 12 mM or by the depolarizations of Em of up to 60 mV that sometimes occurred in low or zero [Ca]o. 6. Only 2\u2010\u20103 mM\u2010aClo was sufficient to impede substantially the ready loss of aCli in HEPES\u2010buffered solutions. 7. In high\u2010K solutions (45 mM), Cl appeared to be passively distributed since, at equilibrium, Em and ECl differed by less than 2 mV. 8. In HEPES\u2010buffered Tyrode, ECl of quiescent papillary muscle of the guinea\u2010pig was, on average, 39 mV positive to Em. 9. It is concluded that liquid ion\u2010exchanger Cl\u2010 \u2010sensitive micro\u2010electrodes are suitable for studying the Cl regulation of sheep Prukinje fibres, and probably of other cardiac tissues. The measurements of resting aCli are quite accurate when using either HEPES or bicarbonate\u2010buffered Tyrode. The results are discussed in relation to estimates of the apparent membrane Cl permeability under various conditions and the possible existence of an inwardly directed \u2018Cl pump'.<\/jats:p>","DOI":"10.1113\/jphysiol.1979.sp012956","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:28:50Z","timestamp":1418974130000},"page":"83-109","source":"Crossref","is-referenced-by-count":61,"title":["Non\u2010passive chloride distribution in mammalian heart muscle: micro\u2010electrode measurement of the intracellular chloride activity."],"prefix":"10.1113","volume":"295","author":[{"given":"R D","family":"Vaughan\u2010Jones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1979,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1979.sp012956","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1979.sp012956","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T14:45:26Z","timestamp":1699195526000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1979.sp012956"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,10]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,10]]}},"alternative-id":["10.1113\/jphysiol.1979.sp012956"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1979.sp012956","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,10]]}}}