{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:44:09Z","timestamp":1773661449459,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1982,2,1]],"date-time":"1982-02-01T00:00:00Z","timestamp":381369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1982,2]]},"abstract":"<jats:p>1. Acetylcholine (ACh)\u2010induced currents were studied in completely isolated Lymnaea stagnalis neurones using the voltage\u2010clamp technique. 2. The ACh\u2010activated pathways were shown to be selective for Cl\u2010 ions. 3. It was shown that membrane depolarization inhibits ACh\u2010induced conductance. This phenomenon was called \u2018ACh response inactivation\u2019. 4. Inactivation decreases after lowering the extracellular Ca2+ concentration or after blockade by Mn2+ of the electrically excitable Ca2+ channels. 5. In dialysed neurones an increase of the intracellular Ca2+ concentration inhibits the ACh\u2010induced conductance. 6. The conclusion is made that the inactivation of ACh response by depolarization is initiated by Ca2+ entering the neurone through the electrically excitable Ca channels. 7. The onset and the decay of the ACh response inactivation were studied by analysing the relaxations of the ACh\u2010induced current during and after the application of depolarizing pulses. The most conspicuous relaxation is a slow relaxation observed at the end of a long depolarizing pulse, which appears to reflect the return of the system from the inactivated state to the non\u2010inactivated one. 8. The slow relaxations observed during and after a depolarizing pulse appear correlated with variations of the intracellular Ca2+ concentration, and are distinct from faster relaxations observed in the hyperpolarizing range and attributed to the voltage dependence of the channel open\u2010time.<\/jats:p>","DOI":"10.1113\/jphysiol.1982.sp014058","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:05:22Z","timestamp":1418972722000},"page":"1-19","source":"Crossref","is-referenced-by-count":49,"title":["Inhibition of acetylcholine responses by intracellular calcium in Lymnaea stagnalis neurones."],"prefix":"10.1113","volume":"323","author":[{"given":"N K","family":"Chemeris","sequence":"first","affiliation":[]},{"given":"V N","family":"Kazachenko","sequence":"additional","affiliation":[]},{"given":"A N","family":"Kislov","sequence":"additional","affiliation":[]},{"given":"A L","family":"Kurchikov","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1982,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1982.sp014058","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1982.sp014058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T04:47:26Z","timestamp":1699246046000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1982.sp014058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,2]]}},"alternative-id":["10.1113\/jphysiol.1982.sp014058"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1982.sp014058","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,2]]}}}