{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:21:15Z","timestamp":1761744075059},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1982,10,1]],"date-time":"1982-10-01T00:00:00Z","timestamp":402278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1982,10]]},"abstract":"<jats:p>1. The kinetics of the anomalous K current produced by blocking cations Na, Cs and Sr were analysed by single channel recording in the tunicate egg cell.<\/jats:p><jats:p>2. The open\u2014close kinetics in a single channel with the presence of blocking cation were consistent with the blocking kinetics of the total anomalous K current. The open\u2014close kinetics in a single anomalous K rectifier channel could be regarded as the first order transition between the open and closed states.<\/jats:p><jats:p>3. The closing rate constants of the single channel for the first order transition increased almost linearly on a semilogarithmic scale as the membrane potential became more negative, while the opening rate constants decreased and then increased, showing the minimum at a certain potential level. The latter indicated that the channel was released from the blocking by excessive hyperpolarization.<\/jats:p><jats:p>4. The opening rate constant increased in spite of the fixed concentration of the blocking cation (10 \u03bcM\u2010Cs), when the external K concentration was raised from 200 mM to 400 mM. This result suggested that there are interactions within the channel between the blocking cation and the K ion.<\/jats:p><jats:p>5. When there were two kinds of blocking cations such as Na and Cs, the kinetic properties in a single channel indicated that the two kinds of blocking cations blocked the single channel independently.<\/jats:p><jats:p>6. The single channel conductance of the anomalous K rectifier in 200 mM\u2010Na\u2010containing 200 mM\u2010K solutions was about 1\u00b75 times larger than that in Na\u2010free 200 mM\u2010K solutions.<\/jats:p><jats:p>7. The single channel conductances in 100, 200 and 400 mM\u2010K solution with 10 \u03bcM\u2010Cs were 5\u00b70, 7\u00b71 and 12\u00b73 pS respectively, being roughly proportional to the square root of the K concentration.<\/jats:p><jats:p>8. The density of the anomalous K rectifier channel in the tunicate egg cell was 0\u00b7039\/\u03bcm<jats:sup>2<\/jats:sup>.<\/jats:p><jats:p>9. The amplitude of the single channel current increased with the rise of temperature, the <jats:italic>Q<\/jats:italic><jats:sub>10<\/jats:sub> being 1\u00b75.<\/jats:p>","DOI":"10.1113\/jphysiol.1982.sp014374","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:02:28Z","timestamp":1418972548000},"page":"311-331","source":"Crossref","is-referenced-by-count":61,"title":["Blocking kinetics of the anomalous potassium rectifier of tunicate egg studied by single channel recording"],"prefix":"10.1113","volume":"331","author":[{"given":"Yasuo","family":"Fukushima","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1982,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1982.sp014374","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1982.sp014374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T21:20:57Z","timestamp":1699219257000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1982.sp014374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,10]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,10]]}},"alternative-id":["10.1113\/jphysiol.1982.sp014374"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1982.sp014374","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,10]]}}}