{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:52:15Z","timestamp":1760100735030},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1983,7,1]],"date-time":"1983-07-01T00:00:00Z","timestamp":425865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1983,7]]},"abstract":"<jats:p>Primary cardiac cell cultures were prepared from the hearts of neonatal rats. The patch\u2010clamp method (Hamill, Marty, Neher, Sakmann &amp; Sigworth, 1981) was applied for studying whole\u2010cell Na+ currents and single\u2010channel Na+ currents, respectively. Whole\u2010cell recordings yielded voltage\u2010 and time\u2010dependent Na+ currents which could be blocked by tetrodotoxin. Single\u2010channel Na+ currents were directly compared in cell\u2010attached patches and in inside\u2010out patches. In cell\u2010attached patches the elementary current was about \u20101 pA at \u201010 mV and the slope conductance over a 50 mV voltage range was 15.1 +\/\u2010 1.6 pS (mean +\/\u2010 S.D.). Inactivation during depolarization and after conditioning clamp steps, in the steady state, resulted from a reduced opening probability of Na+ channels. In inside\u2010out patches, with identical solutions at both membrane surfaces, there was a large (40\u201050 mV) shift of channel opening and inactivation kinetics towards more negative potentials. However, for levels of comparable opening probabilities, mean open times of Na+ channels were similar in cell\u2010attached and inside\u2010out patches. Tetrodotoxin (10\u201020 microM) had no effect on Na+ channels when applied from the inside, but blocked them completely after application to the outside membrane surface.<\/jats:p>","DOI":"10.1113\/jphysiol.1983.sp014768","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:56:12Z","timestamp":1418972172000},"page":"389-401","source":"Crossref","is-referenced-by-count":93,"title":["Sodium channels in cultured cardiac cells."],"prefix":"10.1113","volume":"340","author":[{"given":"A B","family":"Cachelin","sequence":"first","affiliation":[]},{"given":"J E","family":"De Peyer","sequence":"additional","affiliation":[]},{"given":"S","family":"Kokubun","sequence":"additional","affiliation":[]},{"given":"H","family":"Reuter","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1983,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1983.sp014768","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1983.sp014768","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T04:44:22Z","timestamp":1697777062000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1983.sp014768"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1983,7]]}},"alternative-id":["10.1113\/jphysiol.1983.sp014768"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1983.sp014768","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,7]]}}}