{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T07:56:40Z","timestamp":1759564600099},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,3,1]],"date-time":"1984-03-01T00:00:00Z","timestamp":446947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1984,3]]},"abstract":"<jats:p>THe current suppressed by light, the generator current, was studied in solitary salamander (Ambystoma tigrinum) rod photoreceptors with the single\u2010micropipette voltage\u2010clamp technique. The effects of Ca, cyclic GMP, and voltage were measured while voltage\u2010 and Ca\u2010activated currents of the inner segment were blocked with Co, Cs, and TEA (tetraethylammonium). The generator current was increased more than 5\u2010fold by lowering the external Ca concentration from 1.5 mM to 10 microM. The generator current could be decreased approximately 1\/2 by injecting Ca into an outer segment. Injection of EGTA quickly increased the generator current approximately 2\u2010fold. After injection ceased, the increase was quickly reversed. The generator current could be increased more than 5\u2010fold by injecting cyclic GMP or 8Br\u2010cyclic GMP. Injection of protons, the pH buffer bicine (N,N\u2010bis[2\u2010hydroxyethyl]glycine), or GMP did not produce a change in the generator current. The current\u2010voltage curve for the generator current was influenced by external Co: in 3 mM\u2010Co the current\u2010voltage curve had a negative resistance between \u201045 and \u201090 mV; in 0.1 mM\u2010Co the current\u2010voltage curve paralleled the voltage axis between \u201045 and \u201090 mV. The difference is attributed to a voltage\u2010dependent block by Co. Susceptibility to the blocking action of Co was reduced by lowering internal or external Ca concentration, or by injecting cyclic GMP. When rods were bathed in a medium containing 7\u2010100 microM\u2010Ca, a step depolarization produced a time\u2010dependent decline in current. Because the reversal potential remained constant, the decline is attributed to an inactivation. The extent of inactivation was reduced by increasing the concentration of external Ca or injecting cyclic GMP.<\/jats:p>","DOI":"10.1113\/jphysiol.1984.sp015131","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:50:56Z","timestamp":1418971856000},"page":"645-664","source":"Crossref","is-referenced-by-count":47,"title":["Control of the generator current in solitary rods of the Ambystoma tigrinum retina."],"prefix":"10.1113","volume":"348","author":[{"given":"P R","family":"MacLeish","sequence":"first","affiliation":[]},{"given":"E A","family":"Schwartz","sequence":"additional","affiliation":[]},{"given":"M","family":"Tachibana","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1984,3]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1984.sp015131","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1984.sp015131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T07:32:40Z","timestamp":1697787160000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1984.sp015131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,3]]}},"alternative-id":["10.1113\/jphysiol.1984.sp015131"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1984.sp015131","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,3]]}}}