{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:39:19Z","timestamp":1773661159685,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,5,1]],"date-time":"1984-05-01T00:00:00Z","timestamp":452217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1984,5]]},"abstract":"<jats:p>The ionic currents in enzymatically isolated vestibular hair cells of the chick were studied by a whole\u2010cell\u2010clamp variation of the patch voltage clamp, and by single channel recording. At membrane potentials more negative than \u201080 mV the hair cell showed anomalous rectification, and at potentials more positive than \u201040 mV large outward K currents were observed in normal saline. The outward K current decreased at large positive potentials, showing an N\u2010shaped I\u2010V relation. The outward K current was carried mostly through the Ca\u2010activated K channel. K currents through the anomalous rectifier channel showed a decay in normal saline. This decay was eliminated reversibly in Na\u2010free saline when the isotonic KCl\u2010EGTA solution was used as the internal medium. However, a fast decay was still observed in Na\u2010free high\u2010K external solution when isotonic CaCl\u2010EGTA was used as the internal medium. An increase in [K]o decreased the decay rate of the inward K current. The single\u2010channel conductance of the anomalous rectifier channel was 50 pS in 160 mM\u2010K saline and 23 pS in 40 mM\u2010K saline. In 100 mM\u2010Ca, \u2010Sr and \u2010Ba salines a large inward current was observed. At positive potentials the inward current carried by Ca and Sr ions showed significant decay; the current became outward at large positive potentials. Since the decay of the inward current was eliminated when 100 microM\u2010quinine was added to the bathing medium, it was probably due to the activation of some Ca\u2010activated K conductance which remained even with isotonic CsCl\u2010EGTA internal medium. The activation kinetics of the Ca channel were studied in 100 mM\u2010Ba solution at low temperatures (9\u201013 degrees C). From a comparison of the time constants of activation with the time constants of the tail currents, it was concluded that the Ca channel follows Hodgkin\u2010Huxley\u2010type m2 kinetics. A slow component that deviated from m2 kinetics was frequently observed at relatively large positive potentials. The steady\u2010state fluctuations of Ba current showed a power density spectrum reasonably well fitted by a sum of two Lorentzian functions. The spectrum has a low\u2010frequency component which indicates kinetics close to the macroscopic activation process of the Ca channel and a high\u2010frequency component that indicates very fast flickering kinetics operating in the Ca channel.<\/jats:p>","DOI":"10.1113\/jphysiol.1984.sp015218","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:49:57Z","timestamp":1418971797000},"page":"561-581","source":"Crossref","is-referenced-by-count":138,"title":["Studies of ionic currents in the isolated vestibular hair cell of the chick."],"prefix":"10.1113","volume":"350","author":[{"given":"H","family":"Ohmori","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1984,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1984.sp015218","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1984.sp015218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T18:51:06Z","timestamp":1697827866000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1984.sp015218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,5]]}},"alternative-id":["10.1113\/jphysiol.1984.sp015218"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1984.sp015218","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,5]]}}}